Detection of biomolecules from solutions with high concentration of salts using probe electrospray and nano-electrospray ionization mass spectrometry

被引：68

作者：

Mandal, Mridul Kanti ^{[1
]}

Chen, Lee Chuin ^{[2
]}

Hashimoto, Yutaka ^{[1
]}

Yu, Zhan ^{[1
,3
]}

Hiraoka, Kenzo ^{[1
]}

机构：

[1] Univ Yamanashi, Clean Energy Res Ctr, Yamanashi 4008511, Japan

[2] Univ Yamanashi, Interdisciplinary Grad Sch Med & Engn, Yamanashi 4008511, Japan

[3] Shenyang Normal Univ, Coll Chem & Biol, Shenyang 110034, Peoples R China

来源：

ANALYTICAL METHODS | 2010年 / 2卷 / 12期

基金：

日本科学技术振兴机构;

关键词：

BIOLOGICAL SAMPLES; SOLID NEEDLE; PROTEINS; MS; NANOELECTROSPRAY; PEPTIDES; WIRE; ESI;

D O I：

10.1039/c0ay00530d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Probe electrospray ionization (PEST) is a recently developed ionization technique which uses a solid needle or wire as sampling probe and ESI emitter instead of capillary. PESI is free from the clogging problem and it has high tolerance to salts and urea. We present herein a comparative study of the probe electrospray ionization (PESI) and nano-electrospray ionization (nano-ESI) for the measurement of biomolecules in the sample solutions with high concentrations of salts and urea. Our results show that PESI could provide equivalent ionization performance with nano-ESI, and in certain cases it could be superior to nano-ESI for the samples with high concentration (> 100 mM) of salts and urea. Therefore, PESI can be useful for the direct analysis of cell/tissue extracts and protein digestion without or reduced procedures in sample purification.

引用

页码：1905 / 1912

页数：8

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