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2-Gy whole-body irradiation significantly alters the balance of CD4+CD25- T effector cells and CD4+CD25+Foxp3+ T regulatory cells in mice
被引:56
作者:
Qu, Yanyan
[1
]
Zhang, Baojun
[1
]
Liu, Shuchun
[2
]
Zhang, Aijun
[3
]
Wu, Tingting
[1
]
Zhao, Yong
[1
]
机构:
[1] Chinese Acad Sci, Transplantat Biol Res Div, State Key Lab Biomembrane & Membrane Biotechnol, Inst Zool, Beijing 100101, Peoples R China
[2] Jilin Univ, Key Lab Radiobiol, Minist Hlth, Sch Publ Hlth, Changchun 130023, Peoples R China
[3] Shandong Univ, Qilu Hosp, Dept Hematol, Jinan 250100, Peoples R China
关键词:
CD4(+)CD25(+)Foxp3(+) Treg cells;
Foxp3;
gamma-ray irradiation;
immune tolerance;
IMMUNOLOGICAL SELF-TOLERANCE;
TRANSCRIPTION FACTOR FOXP3;
GAMMA-IRRADIATION;
MIXED CHIMERISM;
TRANSPLANTATION;
REQUIREMENT;
EXPRESSION;
INDUCTION;
APOPTOSIS;
MOUSE;
D O I:
10.1038/cmi.2010.45
中图分类号:
R392 [医学免疫学];
Q939.91 [免疫学];
学科分类号:
100102 ;
摘要:
CD4(+)CD25(+) T regulatory (Treg) cells are critical in inducing and maintaining immunological self-tolerance as well as transplant tolerance. The effect of low doses of whole-body irradiation (WBI) on CD4(+)CD25(+)Foxp3(+) Treg cells has not been determined. The proportion, phenotypes and function of CD4(+)CD25(+) Treg cells were investigated 0.5, 5 and 15 days after euthymic, thymectomized or allogeneic bone marrow transplanted C57BL/6 mice received 2-Gy gamma-rays of WBI. The 2-Gy WBI significantly enhanced the ratios of CD4(+)CD25(+) Treg cells and CD4(+)CD25(+)Foxp3(+) Treg cells to CD4(+) T cells in peripheral blood, lymph nodes, spleens and thymi of mice. The CD4(+)CD25(+) Treg cells of the WBI-treated mice showed immunosuppressive activities on the immune response of CD4(+)CD25(-) T effector cells to alloantigens or mitogens as efficiently as the control mice. Furthermore, 2-Gy gamma-ray WBI significantly increased the percentage of CD4(+)CD25(+)Foxp3(+) Treg cells in the periphery of either thymectomized mice or allogeneic bone marrow transplanted mice. The in vitro assay showed that ionizing irradiation induced less cell death in CD4(+)CD25(+)Foxp3(+) Treg cells than in CD4(+)CD25(-) T cells. Thus, a low dose of WBI could significantly enhance the level of functional CD4(+)CD25(+)Foxp3(+) Treg cells in the periphery of naive or immunized mice. The enhanced proportion of CD4(+)CD25(+)Foxp3(+) Treg cells in the periphery by a low dose of WBI may make hosts more susceptible to immune tolerance induction. Cellular & Molecular Immunology (2010) 7, 419-427; doi: 10.1038/cmi.2010.45; published online 27 September 2010
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页码:419 / 427
页数:9
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