Detection of cellular H2O2 in living cells based on horseradish peroxidase at the interface of Au nanoparticles decorated graphene oxide

被引：50
作者：
Yu, Chunmei [1 ]
Wang, Li [1 ]
Li, Weibo [1 ]
Zhu, Chun [2 ]
Bao, Ning [1 ]
Gu, Haiying [1 ]
机构：
[1] Nantong Univ, Sch Publ Hlth, Nantong 226019, Peoples R China
[2] Nantong Univ, Xinglin Coll, Nantong 226008, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2015年 / 211卷
基金：
中国国家自然科学基金;
关键词：
Graphene oxide; Horseradish peroxidase; Hydrogen peroxide; Living cells; Biosensor; NITROGEN-DOPED GRAPHENE; HYDROGEN-PEROXIDE; DIRECT ELECTROCHEMISTRY; FUNCTIONALIZED-GRAPHENE; OXIDATIVE STRESS; CANCER-CELLS; BIOSENSOR; CARBON; IMMOBILIZATION; ELECTRODE;
D O I：
10.1016/j.snb.2015.01.064
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An interface of graphene oxide (GO) decorated with Au nanoparticles (AuNPs) was fabricated to immobilize horseradish peroxidase (HRP) for amperometric detection of H2O2. Due to the biocompatibility of AuNPs and large surface area of GO, the immobilized HRP could retain its inherent bioactivity and exhibited remarkable performance for H2O2 detection. These remarkable analytical advantages such as low detection limit, wide linear range and fast response time could fulfill the requirement of real-time measurement of cellular H2O2 in living cells. Interestingly, results indicated that the levels of intracellular H2O2 depended on the types of cells. This approach provided a simple and effective platform for sensing H2O2 inside biological cells and could be used for the study of H2O2-involved dynamic biological processes. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：17 / 24
页数：8
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