Multilayered silk scaffolds for meniscus tissue engineering

被引：148

作者：

Mandal, Biman B. ^{[1
]}

Park, Sang-Hyug ^{[1
]}

Gil, Eun S. ^{[1
]}

Kaplan, David L. ^{[1
]}

机构：

[1] Tufts Univ, Dept Biomed Engn, Medford, MA 02155 USA

来源：

BIOMATERIALS | 2011年 / 32卷 / 02期

关键词：

Silk; Meniscus; Cartilage; Tissue engineering; Biomaterials; MESENCHYMAL STEM-CELLS; IN-VIVO; KNEE; CARTILAGE; DEGRADATION; REPAIR; REGENERATION; MENISCECTOMY; ALGINATE; COLLAGEN;

D O I：

10.1016/j.biomaterials.2010.08.115

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Removal of injured/damaged meniscus a vital fibrocartilaginous load-bearing tissue impairs normal knee function and predisposes patients to osteoarthritis Meniscus tissue engineering solution is one option to improve outcomes and relieve pain In an attempt to fabricate knee meniscus grafts three layered wedge shaped silk meniscal scaffold system was engineered to mimic native meniscus architecture The scaffolds were seeded with human fibroblasts (outside) and chondrocytes (inside) in a spatial separated mode similar to native tissue in order to generate meniscus-like tissue in vitro In chondrogenic culture in the presence of TGF-b3 cell-seeded constructs increased in cellularity and extracellular matrix (ECM) content Histology and Immunohistochemistry confirmed maintenance of chondrocytic phenotype with higher levels of sulfated glycosaminoglycans (sGAG) and collagen types I and II Improved scaffold mechanical properties along with ECM alignment with time in culture suggest this multiporous silk construct as a useful micro-patterned template for directed tissue growth with respect to form and function of meniscus-like tissue (C) 2010 Elsevier Ltd All rights reserved

引用

页码：639 / 651

页数：13

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