Bi-directional modulation of cellular interactions in an in vitro co-culture model of tendon-to-bone interface

被引：15
作者：
Calejo, I. [1 ,2 ]
Costa-Almeida, Raquel [1 ,2 ]
Goncalves, Ana Isabel [1 ,2 ]
Berdecka, Dominika [1 ,2 ]
Reis, Rui Luis [1 ,2 ,3 ]
Gomes, Manuela Estima [1 ,2 ,3 ]
机构：
[1] Univ Minho, Headquarters European Inst Excellence Tissue Engn, Res Grp Biomat Biodegradables & Biomimet 3Bs, Parque Ciencia & Tecnol, Barco, Guimaraes, Portugal
[2] Braga GuimarAes, ICVS, PT Govt Associate Lab 3Bs, Braga, Portugal
[3] Headquarters Univ Minho, Discoveries Ctr Regenerat & Precis Med, Barco, Guimaraes, Portugal
来源：
CELL PROLIFERATION | 2018年 / 51卷 / 06期
基金：
欧盟地平线“2020”;
关键词：
MESENCHYMAL STEM-CELLS; MARROW STROMAL CELLS; CHONDROGENIC DIFFERENTIATION; EXTRACELLULAR-MATRIX; MECHANICAL LOAD; TENASCIN-C; EXPRESSION; REGENERATION; ATTACHMENT; RUNX2;
D O I：
10.1111/cpr.12493
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Objectives This work aimed at studying in vitro interactions between human tendon-derived cells (hTDCs) and pre-osteoblasts (pre-OBs) that may trigger a cascade of events involved in enthesis regeneration. Materials and methods The effect of 5 osteogenic medium (OM) conditions over the modulation of hTDCs and pre-OBs towards the tenogenic and osteogenic phenotypes, respectively, was studied. Three different medium conditions were chosen for subsequently establishing a direct co-culture system in order to study the expression of bone, tendon and interface-related markers. Results A higher matrix mineralization and ALP activity was observed in co-cultures in the presence of OM. Higher transcription levels of bone- (ALPL, RUNX2, SPP1) and interface-related genes (ACAN, COMP) were found in co-cultures. The expression of aggrecan was influenced by the presence of OM and cell-cell interactions occurring in co-culture. Conclusions The present work assessed both the influence of OM on cell phenotype modulation and the importance of co-culture models while promoting cell-cell interactions and the exchange of soluble factors in triggering an interface-like phenotype to potentially modulate enthesis regeneration.
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页数：15
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