Serotype IV Streptococcus agalactiae ST-452 has arisen from large genomic recombination events between CC23 and the hypervirulent CC17 lineages

被引：20

作者：

Campisi, Edmondo ^{[1
,2
]}

Rinaudo, C. Daniela ^{[1
]}

Donati, Claudio ^{[3
]}

Barucco, Mara ^{[1
,4
]}

Torricelli, Giulia ^{[1
]}

Edwards, Morven S. ^{[5
]}

Baker, Carol J. ^{[5
,6
]}

Margarit, Imma ^{[1
]}

Rosini, Roberto ^{[1
]}

机构：

[1] GSK Vaccines Srl, Siena, Italy

[2] Sapienza Univ Roma, Rome, Italy

[3] Fdn Edmund Mach, Res & Innovat Ctr, Dept Computat Biol, San Michele All Adige, Italy

[4] Univ Pisa, Dept Phys Enrico Fermi, Pisa, Italy

[5] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA

[6] Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

GROUP-B STREPTOCOCCUS; ANTIBIOTIC-RESISTANCE; PREGNANT-WOMEN; DISEASE; PROTEIN; IDENTIFICATION; FIBRINOGEN; BINDING; EVOLUTIONARY; EPIDEMIOLOGY;

D O I：

10.1038/srep29799

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Streptococcus agalactiae (Group B Streptococcus, GBS) causes life-threatening infections in newborns and adults with chronic medical conditions. Serotype IV strains are emerging both among carriers and as cause of invasive disease and recent studies revealed two main Sequence Types (STs), ST-452 and ST-459 assigned to Clonal Complexes CC23 and CC1, respectively. Whole genome sequencing of 70 type IV GBS and subsequent phylogenetic analysis elucidated the localization of type IV isolates in a SNP-based phylogenetic tree and suggested that ST-452 could have originated through genetic recombination. SNPs density analysis of the core genome confirmed that the founder strain of this lineage originated from a single large horizontal gene transfer event between CC23 and the hypervirulent CC17. Indeed, ST-452 genomes are composed by two parts that are nearly identical to corresponding regions in ST-24 (CC23) and ST-291 (CC17). Chromosome mapping of the major GBS virulence factors showed that ST-452 strains have an intermediate yet unique profile among CC23 and CC17 strains. We described unreported large recombination events, involving the cps IV operon and resulting in the expansion of serotype IV to CC23. This work sheds further light on the evolution of GBS providing new insights on the recent emergence of serotype IV.

引用

页数：11

共 60 条

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