Expression of human growth hormone gene in Pleurotus eryngii

被引：4
|
作者：
Kim, Seung [1 ]
Sapkota, Kumar [2 ]
Choi, Bong-Suk [2 ,3 ]
Kim, Sung-Jun [2 ,3 ]
机构：
[1] Gwangju Univ, Dept Alternat Med, Kwangju 503703, South Korea
[2] Chosun Univ, Dept Biotechnol, Kwangju 501759, South Korea
[3] Chosun Univ, Res Team Prot Act Control BK21, Kwangju 501759, South Korea
来源：
CENTRAL EUROPEAN JOURNAL OF BIOLOGY | 2010年 / 5卷 / 06期
关键词：
Agrobacterium tumefaciens; Human growth hormone; Pleurotus eryngii; Gene transfer; Gene expression; AGROBACTERIUM-MEDIATED TRANSFORMATION; T-DNA; TUMEFACIENS; SYSTEM;
D O I：
10.2478/s11535-010-0071-8
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Recombinant human growth hormone (rhGH) has been widely used in many medical applications. Large scale production of rhGH is important for a wider application of this molecule in the field of proteomics. This investigation reports a modified Agrobacterium-mediated method for transfer and expression of human growth hormone gene in the popular edible mushroom Pleurotus eryngii. A binary vector pCAMBIA1304 containing the hGH2 gene was constructed and introduced into Pleurotus eryngii via Agrobacterium tumefaciens-mediated transformation. Integration of hGH2 into mushroom genome was detected by PCR and expression was confirmed by Western blot assay. The successful expression of the human growth hormone gene in mushroom suggests that the proposed modified transformation system is probably useful for the production of transgenic mushrooms.
引用
收藏
页码：791 / 799
页数：9
相关论文
共 50 条
  • [41] EXPRESSION OF THE GENE ENCODING GROWTH-HORMONE IN THE HUMAN MAMMARY-GLAND
    MOL, JA
    HENZENLOGMANS, SC
    HAGEMAN, P
    MISDORP, W
    BLANKENSTEIN, MA
    RIJNBERK, A
    JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1995, 80 (10): : 3094 - 3096
  • [42] Characterization of Growth Hormone Receptor (GHR) Gene Expression in Human Adipose Tissue
    Glad, Camilla A. M.
    Svensson, Per-Arne
    Nystrom, Fredrik H.
    Jacobson, Peter
    Carlsson, Lena M. S.
    Johannsson, Gudmundur
    Andersson-Assarsson, Johanna C.
    ENDOCRINE REVIEWS, 2014, 35 (03)
  • [43] Gene expression of the human growth hormone locus in the placentas of growth restricted and normally grown fetuses
    Moldenhauer, J
    Platts, A
    Robert, G
    Martins, R
    Krawetz, S
    AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2005, 193 (06) : S139 - S139
  • [44] Transcriptomics Analysis of Primordium Formation in Pleurotus eryngii
    Ye, Dou
    Du, Fang
    Zou, Yajie
    Hu, Qingxiu
    GENES, 2021, 12 (12)
  • [45] Effect of drying temperature on quality of Pleurotus eryngii
    You, Chu-Zhen
    Lin, Jun-Fang
    Guo, Li-Qiong
    Sun, Ping
    Bai, Wei-Fang
    Modern Food Science and Technology, 2013, 29 (06) : 1252 - 1254
  • [46] ANTITUMOR AND IMMUNOMODULATORY ACTIVITY OF PLEUROTUS ERYNGII EXTRACT
    Xue, Zhaohui
    Zhai, Lijuan
    Yu, Wancong
    Wang, Hua
    Kou, Xiaohong
    Peng, Lvyang
    Hu, Dongmei
    JOURNAL OF FOOD BIOCHEMISTRY, 2015, 39 (01) : 19 - 27
  • [47] FRUCTIFICATION VARIABILITY OF CULTIVATED PLEUROTUS-ERYNGII
    CAILLEUX, R
    DIOP, A
    SLEZEC, AM
    JOLY, P
    CRYPTOGAMIE MYCOLOGIE, 1980, 1 (02) : 119 - 138
  • [48] OCCURRENCE OF PANTOEA BEIJINGENSIS ON PLEUROTUS ERYNGII IN CHINA
    Ma, Y.
    Liu, Y.
    Wang, S.
    Zhang, D.
    Zhao, S.
    Ma, K.
    Xu, F.
    JOURNAL OF PLANT PATHOLOGY, 2014, 96 (02) : 433 - 433
  • [49] Effect of culture temperature on the heterologous expression of Pleurotus eryngii versatile peroxidase in Aspergillus hosts
    Eibes, G. M.
    Lu-Chau, T. A.
    Ruiz-Duenas, F. J.
    Feijoo, G.
    Martinez, M. J.
    Martinez, A. T.
    Lema, J. M.
    BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2009, 32 (01) : 129 - 134
  • [50] Over expression of human growth hormone
    Argueta, J
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2003, 225 : U25 - U25
12345