Expression of human growth hormone gene in Pleurotus eryngii

被引：4
|
作者：
Kim, Seung [1 ]
Sapkota, Kumar [2 ]
Choi, Bong-Suk [2 ,3 ]
Kim, Sung-Jun [2 ,3 ]
机构：
[1] Gwangju Univ, Dept Alternat Med, Kwangju 503703, South Korea
[2] Chosun Univ, Dept Biotechnol, Kwangju 501759, South Korea
[3] Chosun Univ, Res Team Prot Act Control BK21, Kwangju 501759, South Korea
来源：
CENTRAL EUROPEAN JOURNAL OF BIOLOGY | 2010年 / 5卷 / 06期
关键词：
Agrobacterium tumefaciens; Human growth hormone; Pleurotus eryngii; Gene transfer; Gene expression; AGROBACTERIUM-MEDIATED TRANSFORMATION; T-DNA; TUMEFACIENS; SYSTEM;
D O I：
10.2478/s11535-010-0071-8
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Recombinant human growth hormone (rhGH) has been widely used in many medical applications. Large scale production of rhGH is important for a wider application of this molecule in the field of proteomics. This investigation reports a modified Agrobacterium-mediated method for transfer and expression of human growth hormone gene in the popular edible mushroom Pleurotus eryngii. A binary vector pCAMBIA1304 containing the hGH2 gene was constructed and introduced into Pleurotus eryngii via Agrobacterium tumefaciens-mediated transformation. Integration of hGH2 into mushroom genome was detected by PCR and expression was confirmed by Western blot assay. The successful expression of the human growth hormone gene in mushroom suggests that the proposed modified transformation system is probably useful for the production of transgenic mushrooms.
引用
收藏
页码：791 / 799
页数：9
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