Live-cell super-resolution imaging with trimethoprim conjugates

被引：0
|
作者：
Wombacher, Richard [1 ]
Heidbreder, Meike [2 ]
van de Linde, Sebastian [3 ]
Sheetz, Michael P. [4 ]
Heilemann, Mike [2 ]
Cornish, Virginia W. [1 ]
Sauer, Markus [3 ]
机构：
[1] Columbia Univ, Dept Chem, New York, NY 10027 USA
[2] Univ Bielefeld, Bielefeld, Germany
[3] Julius Maximilians Univ Wuerzburg, Dept Biotechnol & Biophys, Wurzburg, Germany
[4] Columbia Univ, Dept Biol Sci, New York, NY 10027 USA
来源：
NATURE METHODS | 2010年 / 7卷 / 09期
基金：
美国国家卫生研究院; 美国国家科学基金会;
关键词：
MICROSCOPY; DEPLETION; NANOSCOPY;
D O I：
10.1038/NMETH.1489
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The spatiotemporal resolution of subdiffraction fluorescence imaging has been limited by the difficulty of labeling proteins in cells with suitable fluorophores. Here we report a chemical tag that allows proteins to be labeled with an organic fluorophore with high photon flux and fast photoswitching performance in live cells. This label allowed us to image the dynamics of human histone H2B protein in living cells at similar to 20 nm resolution.
引用
收藏
页码：717 / 719
页数：3
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