Selectively screen the antibacterial peptide from the hydrolysates of highland barley

被引：24

作者：

Pei, Jinjin ^{[1
,2
,3
]}

Feng, Zhenzhen ^{[1
]}

Ren, Ting ^{[1
]}

Jin, Wengang ^{[1
]}

Li, Xinsheng ^{[1
]}

Chen, Dejing ^{[1
]}

Tao, Yanduo ^{[2
,3
]}

Dang, Jun ^{[2
,3
]}

机构：

[1] Shaanxi Univ Technol, Shaanxi Key Lab Biol & Bioresources, Chaoyang Rd, Hanzhogn 723001, Shaanxi, Peoples R China

[2] Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China

[3] Qinghai Key Lab Tibetan Med Res, Xining, Qinghai, Peoples R China

来源：

ENGINEERING IN LIFE SCIENCES | 2018年 / 18卷 / 01期

关键词：

Antibacterial peptide; Characterization; Highland barley; Magnetic liposome; Purification; ANTIMICROBIAL PEPTIDES; BACTERIOCIN; THERMODYNAMICS; CONSEQUENCES; PURIFICATION; BINDING;

D O I：

10.1002/elsc.201700118

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Highland barley is one of the most important industrial crops in Tibetan plateau. Previous research indicated that highland barley has many medical functions. In this work, the antibacterial abilities of highland barley were investigated. The protein solutions hydrolyzed by trypsin for 4 h exhibited the highest antibacterial activity. An antibacterial peptide, barleycin, was screened and purified by magnetic liposome extraction combining with the protein profiles of reversed-phase high-performance liquid chromatography (RP-HPLC). Structure, characterization, and safety evaluation of barleycin were further investigated. Amino acids sequence was determined as Lys-Ile-Ile-Ile-Pro-Pro-Leu-Phe-His by N-sequencing. Circular dichroism spectra indicated the a-helix conformation of barleycin. The activity spectrum included Bacillus subtilis, Staphylcoccus aureus, Listeria innocua and Escherichia coli and the MICs were from 4 to 16g/mL. Safety evaluations with cytotoxicity and hemolytic suggested this antibacterial peptide could be considered as safe at MICs. Finally, mode of action of barleycin on sensitive cells was primarily studied. The results suggested the damage of cell membrane.

引用

页码：48 / 54

页数：7

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