Purification and Characterization of Peroxidase from Papaya (Carica papaya) Fruit

被引：33

作者：

Pandey, Veda P. ^{[1
]}

Singh, Swati ^{[1
]}

Singh, Rupinder ^{[1
]}

Dwivedi, Upendra N. ^{[1
]}

机构：

[1] Univ Lucknow, Dept Biochem, Lucknow 226007, Uttar Pradesh, India

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2012年 / 167卷 / 02期

关键词：

Fruit ripening; Peroxidase; Real-time PCR; Papaya; Salicylic acid; CLASS-III PEROXIDASES; CATIONIC PEROXIDASE; ISOENZYME; OXIDASE; ACID;

D O I：

10.1007/s12010-012-9672-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ripening of papaya fruit was found to be characterized with a decrease in peroxidase activity and its transcript. This peroxidase was purified to homogeneity through successive steps of ammonium sulfate fractionation, ion exchange and molecular exclusion chromatography. The peroxidase was purified 30.22-folds with overall recovery of 44.37 % and specific activity of 68.59. Purified peroxidase was found to be a heterotrimer of similar to 240 kDa, containing two subunits each of 85 and one of 70 kDa. Purified enzyme exhibited pH and temperature optima of 7.0 and 40 A degrees C, respectively. (m) values for substrates -dianicidin, guaiacol and ascorbic acid were found to be 0.125, 0.8 and 5.2 mM, respectively. (m) for H2O2 was found to be 0.25 mM. Salicylic acid was found to activate peroxidase up to 50 mu M concentration, beyond which it acted as inhibitor. Ca2+ and Mg2+ activated peroxidase while sodium azide, SDS, and Triton X-100 were found to inhibit peroxidase.

引用

页码：367 / 376

页数：10

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