The first crop plant genetically engineered to release an insect pheromone for defence

被引：128

作者：

Bruce, Toby J. A. ^{[1
]}

Aradottir, Gudbjorg I. ^{[1
]}

Smart, Lesley E. ^{[1
]}

Martin, Janet L. ^{[1
]}

Caulfield, John C. ^{[1
]}

Doherty, Angela ^{[1
]}

Sparks, Caroline A. ^{[1
]}

Woodcock, Christine M. ^{[1
]}

Birkett, Michael A. ^{[1
]}

Napier, Johnathan A. ^{[1
]}

Jones, Huw D. ^{[1
]}

Pickett, John A. ^{[1
]}

机构：

[1] Rothamsted Res, Harpenden AL5 2JQ, Herts, England

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

英国生物技术与生命科学研究理事会;

关键词：

APHID ALARM PHEROMONE; TERPENOID METABOLISM; BIOSYNTHETIC-PATHWAY; TRANSFORMATION; SYNTHASE; TOMATO;

D O I：

10.1038/srep11183

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Insect pheromones offer potential for managing pests of crop plants. Volatility and instability are problems for deployment in agriculture but could be solved by expressing genes for the biosynthesis of pheromones in the crop plants. This has now been achieved by genetically engineering a hexaploid variety of wheat to release (E)-beta-farnesene (E beta f), the alarm pheromone for many pest aphids, using a synthetic gene based on a sequence from peppermint with a plastid targeting amino acid sequence, with or without a gene for biosynthesis of the precursor farnesyl diphosphate. Pure E beta f was produced in stably transformed wheat lines with no other detectable phenotype but requiring targeting of the gene produced to the plastid. In laboratory behavioural assays, three species of cereal aphids were repelled and foraging was increased for a parasitic natural enemy. Although these studies show considerable potential for aphid control, field trials employing the single and double constructs showed no reduction in aphids or increase in parasitism. Insect numbers were low and climatic conditions erratic suggesting the need for further trials or a closer imitation, in the plant, of alarm pheromone release.

引用

页数：9

共 26 条

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Agriculture and Horticulture Development Board, 2014, HGCA INF SHEET, V32

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