Highly selective and sensitive DNA measurement by micro electrical impedance spectroscopy

被引：0

作者：

Lee, SW ^{[1
]}

Yamamoto, T ^{[1
]}

Fujii, T ^{[1
]}

机构：

[1] Univ Tokyo, Inst Ind Sci, Meguro Ku, Tokyo 1538505, Japan

来源：

Transducers '05, Digest of Technical Papers, Vols 1 and 2 | 2005年

关键词：

electrical impedance spectroscopy; mu-; EIS; microfluidics;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

A novel mu-EIS (micro electrical impedance spectroscopy) device for label free analysis of DNA are developed and experimentally demonstrated. The feature of mu-EIS system is not only detecting DNA without any labelling, but also controlling the conformation that would enhance the EIS signal. The mu-EIS system is fully integrated with a microfluidic network made of polydimethylsiloxane (PDMS), a micro measurement electrode and a micropump in order to conduct a semiautomated measurement controlled by an external PC system. The micro EIS system is capable of detecting the DNA concentration of a few nM in aqueous solution of a few pl in volume by virtue of the conformation enhanced impedance response. As a first demonstration of conformational change induced DNA analysis, the frequency and the electric field strength dependence of various length and concentrations of DNA were evaluated.

引用

页码：1314 / 1317

页数：4

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