Evaluation of the Premi®Test Salmonella, a commercial low-density DNA microarray system intended for routine identification and typing of Salmonella enterica

被引：44

作者：

Wattiau, Pierre ^{[1
]}

Weijers, Thijs ^{[2
]}

Andreoli, Peter ^{[2
]}

Schliker, Christine ^{[1
]}

Veken, Heidi Vander ^{[1
]}

Maas, Henny M. E. ^{[3
]}

Verbruggen, Anjo J. ^{[3
]}

Heck, Max E. O. C. ^{[3
]}

Wannet, Wim J. ^{[3
]}

Imberechts, Hein ^{[1
]}

Vos, Pieter ^{[2
]}

机构：

[1] Vet & Agrochem Res Ctr, Dept Bacteriol & Immunol, B-1180 Brussels, Belgium

[2] Check Points bv, NL-9708 PW Wageningen, Netherlands

[3] Natl Inst Publ Hlth & Environm, RIVM, Microbiol Lab Hlth Protect, NL-3720 BA Bilthoven, Netherlands

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2008年 / 123卷 / 03期

关键词：

Salmonella; microarray; LDR; multiplex amplification; serotyping; genotyping;

D O I：

10.1016/j.ijfoodmicro.2008.01.006

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A new commercial system based on genetic profiling and aimed at identifying Salmonella enterica serovars was evaluated by comparing its performance with classical serotyping on 443 strains. Within 62 serovars represented, 60 gave unique genetic profiles while 2 were undistinguishable. Results were obtained within 8 h, were reproducible and clear-cut. The system allowed single-tube processing of the samples and required no peculiar technical skill. It showed interesting potential for routine laboratory testing. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：293 / 298

页数：6

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