IgG Avidity in Samples Collected on Filter Paper: Importance of The Early Diagnosis of Congenital Toxoplasmosis

被引:1
作者
de Souza, Jessica Yonara [1 ]
Gomes, Taynara Cristina [1 ]
Alves Rezende, Hanstter Hallison [1 ]
Storchilo, Heloisa Ribeiro [1 ]
Rodrigues, Patricia Giffron [1 ]
de Castro, Ana Maria [1 ]
机构
[1] Univ Fed Goias, Goiania, Go, Brazil
来源
REVISTA BRASILEIRA DE GINECOLOGIA E OBSTETRICIA | 2021年 / 43卷 / 12期
关键词
toxoplasmosis; IgG avidity; filter paper; neonatal screening; pregnancy; PREGNANT-WOMEN; STATE; RISK;
D O I
10.1055/s-0041-1740272
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective The purpose of the present study is to standardize and evaluate the use of the immunoglobulin G (IgG) antibody avidity test on blood samples from newborns collected on filter paper to perform the heel test aiming at its implementation in ongoing programs. Methods Blood samples from newborns were collected on filter paper simultaneously with the heel prick test. All samples were subjected to immunoglobulin M IgM and IgG enzyme-linked immunosorbent assays (ELISA). Peripheral blood was collected again in the traditional way and on filter paper from newborns with high IgG levels ( (3) 3). Three types of techniques were performed, the standard for measuring IgG in serum, adapted for filter paper and the technique of IgG avidity in serum and on filter paper. The results of the avidity test were classified according to the Rahbari protocol. Results Among the 177 samples, 17 were collected in duplicate from the same child, 1 of peripheral blood and 1 on filter paper. In this analysis, 1 (5.88%) of the 17 samples collected in duplicate also exhibited low IgG avidity, suggesting congenital infection. In addition, the results obtained from serum and filter paper were in agreement, that is, 16 (94.12%) samples presented high avidity, with 100% agreement between the results obtained from serum and from filter paper. Conclusion The results of the present study indicate that the avidity test may be another valuable method for the diagnosis of congenital toxoplasmosis in newborns.
引用
收藏
页码:887 / 893
页数:7
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