In vitro antiviral activity of sulfated Auricularia auricula polysaccharides

被引：121

作者：

The Luong Nguyen ^{[1
]}

Chen, Jin ^{[2
]}

Hu, Yuanliang ^{[1
]}

Wang, Deyun ^{[1
]}

Fan, Yunpeng ^{[1
]}

Wang, Junmin ^{[1
]}

Abula, Saifuding ^{[1
]}

Zhang, Jing ^{[1
]}

Qin, Tao ^{[1
]}

Chen, Xingying ^{[1
]}

Chen, Xiaolan ^{[1
]}

Khakame, Shem Kakai ^{[3
]}

Bao Khanh Dang ^{[4
]}

机构：

[1] Nanjing Agr Univ, Coll Vet Med, Inst Tradit Chinese Vet Med, Nanjing 210095, Jiangsu, Peoples R China

[2] Jiangsu Acad Agr Sci, Natl Res Ctr Vet Biol Engn & Technol, Nanjing 210014, Peoples R China

[3] Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

[4] Univ Montreal, Fac Med Vet, Dept Biomed, St Hyacinthe, PQ J2S 7C6, Canada

来源：

CARBOHYDRATE POLYMERS | 2012年 / 90卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Sulfated Auricularia auricula polysaccharide; Newcastle disease virus; Cellular infectivity; CELLULAR INFECTIVITY; GLUCAN; JUDAE;

D O I：

10.1016/j.carbpol.2012.06.060

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Total Auricularia auricula polysaccharide (AAP(1)) was prepared by extracting and removing the proteins. Column chromatography was used to further graded it into AAP(1) and AAP(2). Three AAPs were modified by chlorosulfonic acid-pyridine method to obtain three sulfated AAPs (sAAPs), sAAP(1), sAAP(1) and sAAP(2). respectively. Three sAAPs and Newcastle disease virus (NDV) were added into cultivation system of chicken embryo fibroblast (CEF) in three manners, pre-, post- and simultaneous-adding polysaccharide with NDV respectively, taking three non-modified AAPs as control. Their anti-viral activities were compared by MIT method. The results showed that sAAPs and AAPs at a certain concentration could significantly inhibit the cellular infectivity of NDV in three manners. The effects of sAAPs were better than that of AAPs. It indicated that sulfated modification could enhance the antiviral activity of AAP. sAAP(1) and sAAP, possessed stronger activity and would be as the component of a new-type antiviral drug. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：1254 / 1258

页数：5

共 38 条

[1] Characterization of the anticoagulant actions of a semisynthetic curdlan sulfate [J].

Alban, S ;

Franz, G .

THROMBOSIS RESEARCH, 2000, 99 (04) :377-388

[2] Anti-HIV-1 activity of low molecular weight sulfated chitooligosaccharides [J].

Artan, Murat ;

Karadeniz, Fatih ;

Karagozlu, Mustafa Zafer ;

Kim, Moon-Moo ;

Kim, Se-Kwon .

CARBOHYDRATE RESEARCH, 2010, 345 (05) :656-662

[3] ANTI-HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ACTIVITY OF SULFATED MONOSACCHARIDES - COMPARISON WITH SULFATED POLYSACCHARIDES AND OTHER POLYIONS [J].

BAGASRA, O ;

WHITTLE, P ;

HEINS, B ;

POMERANTZ, RJ .

JOURNAL OF INFECTIOUS DISEASES, 1991, 164 (06) :1082-1090

[4] Structural features and in vitro antiviral activities of sulfated polysaccharides from Sphacelaria indica [J].

Bandyopadhyaya, Shruti S. ;

Navid, Mojdeh Heidary ;

Ghosh, Tuhin ;

Schnitzler, Paul ;

Ray, Bimalendu .

PHYTOCHEMISTRY, 2011, 72 (2-3) :276-283

[5] A sulfated galactan with antioxidant capacity from the green variant of tetrasporic Gigartina skottsbergii (Gigartinales, Rhodophyta) [J].

Barahona, Tamara ;

Encinas, Maria V. ;

Mansilla, Andres ;

Matsuhiro, Betty ;

Zuniga, Elisa A. .

CARBOHYDRATE RESEARCH, 2012, 347 (01) :114-120

[6] Catalytic synthesis and antitumor activities of sulfated polysaccharide from Gynostemma pentaphyllum Makino [J].

Chen, Tong ;

Li, Bo ;

Li, Yuanyuan ;

Zhao, Chuande ;

Shen, Jianmin ;

Zhang, Haixia .

CARBOHYDRATE POLYMERS, 2011, 83 (02) :554-560

[7] The hypocholesterolemic effect of two edible mushrooms: Auricularia auricula (tree-ear) and Tremella fuciformis (white jelly-leaf) in hypercholesterolemic rats [J].

Cheung, PCK .

NUTRITION RESEARCH, 1996, 16 (10) :1721-1725

[8] INVITRO EVALUATION OF CELL BIOMATERIAL INTERACTION BY MTT ASSAY [J].

CIAPETTI, G ;

CENNI, E ;

PRATELLI, L ;

PIZZOFERRATO, A .

BIOMATERIALS, 1993, 14 (05) :359-364

[9] A NOTE ON DETERMINATION OF ESTER SULPHATE CONTENT OF SULPHATED POLYSACCHARIDES [J].

DODGSON, KS ;

PRICE, RG .

BIOCHEMICAL JOURNAL, 1962, 84 (01) :106-&

[10] Epimedium polysaccharide and propolis flavone can synergistically inhibit the cellular infectivity of NDV and improve the curative effect of ND in chicken [J].

Fan, Yunpeng ;

Liu, Jiaguo ;

Wang, Deyun ;

Hu, Yuanliang ;

Yang, Shujuan ;

Wang, Junmin ;

Guo, Liwei ;

Zhao, Xiaona ;

Wang, Huali ;

Jiang, Yu .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2011, 48 (03) :439-444

← 1 2 3 4 →