Duraplasty of PHBV/PLA/Col membranes promotes axonal regeneration by inhibiting NLRP3 complex and M1 macrophage polarization in rats with spinal cord injury

被引:13
作者
Zhao, Tengfei [1 ]
Xu, Kan [1 ]
Wu, Qionghua [1 ]
Wang, Chenggui [1 ]
Xiao, Shining [1 ]
Li, Heyangzi [2 ,3 ]
He, Teng [2 ,3 ]
Wang, Linlin [2 ,3 ]
Li, Fangcai [1 ]
Chen, Qixin [1 ]
机构
[1] Zhejiang Univ, Affiliated Hosp 2, Sch Med, Dept Orthoped Surg, 88 Jiefang Rd, Hangzhou 310009, Peoples R China
[2] Zhejiang Univ, Dept Basic Med Sci, Sch Med, Hangzhou, Peoples R China
[3] Zhejiang Univ, Sir Run Run Shaw Hosp, Dept Orthopaed, Sch Med, Hangzhou, Peoples R China
基金
中国国家自然科学基金;
关键词
duraplasty after decompression; inflammasome; macrophage; PHBV; PLA; Col membranes; polarization; spinal cord injury; EARLY SURGICAL DECOMPRESSION; DURAL SUBSTITUTE; NANOFIBROUS SCAFFOLDS; NEUROTROPHIC FACTORS; LOCOMOTOR FUNCTION; REPAIR; MODEL; FABRICATION; PRESSURE; EFFICACY;
D O I
10.1096/fj.202000190RR
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Duraplasty after decompression decreases the lesion size and scar formation, promoting better functional recovery, but the underlying mechanism has not been clarified. Here, we fabricated a series of poly(hydroxybutyrate-co-hydroxyvalerate)/polylactic acid/collagen (PHBV/PLA/Col) membranes and cultured them with VSC4.1 motor neurons. The material characteristics and in vitro biological characteristics were evaluated. In the subcutaneous implantation test, PHBV/PLA/COl scaffolds supported the cellular infiltration, microvasculature formation, and decreased CD86-positive macrophage aggregation. Following contusion spinal cord injury at T10 in Sprague-Dawley rats, durotomy was performed with allograft dura mater or PHBV/PLA or PHBV/PLA/Col membranes. At 3 days post-injury, Western blot assay showed decreased the expression of the NLRP3, ASC, cleaved-caspase-1, IL-1 beta, TNF-alpha, and CD86 expression but increased the expression of CD206. Immunofluorescence demonstrated that duraplasty with PHBV/PLA/Col membranes reduced the infiltration of CD86-positive macrophages in the lesion site, decreased the glial fibrillary acidic protein expression, and increased the expression of NF-200. Moreover, duraplasty with PHBV/PLA/Col membranes improved locomotor functional recovery at 8 weeks post-injury. Thus, duraplasty with PHBV/PLA/Col membranes decreased the glial scar formation and promoted axon growth by inhibiting inflammasome activation and modulating macrophage polarization in acute spinal cord injury.
引用
收藏
页码:12147 / 12162
页数:16
相关论文
共 48 条
  • [1] Dural Repair in Cranial Surgery Is Associated with Moderate Rates of Complications with Both Autologous and Nonautologous Dural Substitutes
    Azzam, Daniel
    Romiyo, Prasanth
    Thien Nguyen
    Sheppard, John P.
    Alkhalid, Yasmine
    Lagman, Carlito
    Prashant, Giyarpuram N.
    Yang, Isaac
    [J]. WORLD NEUROSURGERY, 2018, 113 : 244 - 248
  • [2] The Systemic Inflammatory Response after Spinal Cord Injury in the Rat Is Decreased by α4β1 Integrin Blockade
    Bao, Feng
    Omana, Vanessa
    Brown, Arthur
    Weaver, Lynne C.
    [J]. JOURNAL OF NEUROTRAUMA, 2012, 29 (08) : 1626 - 1637
  • [3] Safety and efficacy of the porcine small intestinal submucosa dural substitute: results of a prospective multicenter study and literature review
    Bejjani, Ghassan K.
    Zabramski, Joseph
    [J]. JOURNAL OF NEUROSURGERY, 2007, 106 (06) : 1028 - 1033
  • [4] Moving beyond the glial scar for spinal cord repair
    Bradbury, Elizabeth J.
    Burnside, Emily R.
    [J]. NATURE COMMUNICATIONS, 2019, 10 (1)
  • [5] CEREBROSPINAL FLUID PRESSURE
    BRADLEY, KC
    [J]. JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY, 1970, 33 (03) : 387 - &
  • [6] Does Decompressive Duraplasty Have a Neuroprotective Effect on Spinal Trauma?: An Experimental Study
    Camlar, Mahmut
    Turk, Caglar
    Buhur, Aylin
    Oltulu, Fatih
    Oren, Merve
    Senoglu, Mehmet
    Ozer, Fusun
    [J]. WORLD NEUROSURGERY, 2019, 126 : E288 - E294
  • [7] Rapid co-release of interleukin 1β and caspase 1 in spinal cord inflammation
    Clark, Anna K.
    D'Aquisto, Fulvio
    Gentry, Clive
    Marchand, Fabien
    McMahon, Stephen B.
    Malcangio, Marzia
    [J]. JOURNAL OF NEUROCHEMISTRY, 2006, 99 (03) : 868 - 880
  • [8] Outcomes of 33 patients from the wars in Iraq and Afghanistan undergoing bilateral or bicompartmental craniectomy Clinical article
    Ecker, Robert D.
    Mulligan, Lisa P.
    Dirks, Michael
    Bell, Randy S.
    Severson, Meryl A.
    Howard, Robin S.
    Armonda, Rocco A.
    [J]. JOURNAL OF NEUROSURGERY, 2011, 115 (01) : 124 - 129
  • [9] Spinal duraplasty materials and hydrostasis: a biomechanical study Laboratory investigation
    Finn, Michael A.
    Faulkner, Nathan D.
    Hetzel, Scott J.
    Anderson, Paul A.
    [J]. JOURNAL OF NEUROSURGERY-SPINE, 2011, 15 (04) : 422 - 427
  • [10] Spinal Cord Blood Flow in Patients with Acute Spinal Cord Injuries
    Gallagher, Mathew J.
    Hogg, Florence R. A.
    Zoumprouli, Argyro
    Papadopoulos, Marios C.
    Saadoun, Samira
    [J]. JOURNAL OF NEUROTRAUMA, 2019, 36 (06) : 919 - 929