Chondrogenic potential of stem cells derived from amniotic fluid, adipose tissue, or bone marrow encapsulated in fibrin gels containing TGF-β3

被引：58
作者：
Park, Ji Sun [2 ]
Shim, Myung-Sun [1 ]
Shim, Sung Han [1 ]
Yang, Han Na [2 ]
Jeon, Su Yeon [2 ]
Woo, Dae Gyun [3 ]
Lee, Dong Ryul [1 ]
Yoon, Tae Ki [1 ]
Park, Keun-Hong [2 ]
机构：
[1] CHA Univ, Coll Med, CHA Gangnam Med Ctr, Fertil Ctr, Seoul 135081, South Korea
[2] CHA Univ 606 16, Coll Life Sci, Dept Biomed Sci, Seoul 135081, South Korea
[3] CHA Bio & Diostech Co Ltd, CHA Stem Cell Inst, Seoul 135081, South Korea
来源：
BIOMATERIALS | 2011年 / 32卷 / 32期
关键词：
Fibrin; hMSC; TGF-beta; 3; Chondrogenesis; Cartilage; ENHANCED PROLIFERATION; DIFFERENTIATION; OSTEOGENESIS; REPAIR; SURVIVAL; SCAFFOLD; HEART;
D O I：
10.1016/j.biomaterials.2011.07.043
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
In this study, several types of hMSCs, derived from bone marrow, adipose tissue, or amniotic fluid, were encapsulated in a fibrin hydrogel mixed with TGF-beta 3 and then evaluated for their capacity for differentiation in vitro and in vivo. For determination of stem cell differentiation, RT-PCR, real time quantitative PCR (qPCR), histology, and immunohistochemical assays were used for analysis of chondrogenesis. Using these analysis methods, several of the cultured hMSCS were found to highly express genes and proteins specific to cartilage forming tissues. Additionally, similar trends in expression were found in tissue recovered from nude mice transplanted with several types of hMSCs encapsulated in a fibrin hydrogel containing TGF-beta 3. The results of both in vitro and in vivo analyses showed that cultured or transplanted hMSCs mixed with TGF-beta 3 in a fibrin hydrogel differentiated into chondrocytes, suggesting that these cells would be suitable for reconstruction of hyaline articular cartilage. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：8139 / 8149
页数：11
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