Classical swine fever virus detection: results of a real-time reverse transcription polymerase chain reaction ring trial conducted in the framework of the European network of excellence for epizootic disease diagnosis and control

被引：13

作者：

Hoffmann, Bernd ^{[1
]}

Blome, Sandra ^{[1
]}

Bonilauri, Paolo ^{[2
]}

Fernandez-Pinero, Jovita ^{[3
]}

Greiser-Wilke, Irene ^{[4
]}

Haegeman, Andy ^{[5
]}

Isaksson, Mats ^{[6
]}

Koenen, Frank ^{[5
]}

LeBlanc, Neil ^{[6
]}

Leifer, Immanuel ^{[7
]}

Le Potier, Marie-Frederique ^{[8
]}

Loeffen, Willie ^{[9
]}

Rasmussen, Thomas Bruun ^{[10
]}

Stadejek, Tomasz ^{[11
]}

Stahl, Karl ^{[6
]}

Tignon, Marylene ^{[5
]}

Uttenthal, Ase ^{[10
]}

van der Poel, Wim ^{[9
]}

Beer, Martin ^{[1
]}

机构：

[1] Friedrich Loeffler Inst, Inst Diagnost Virol, D-17493 Greifswald, Riems Island, Germany

[2] Lombardy & Emilia Romagna Expt Zootech Inst, Diagnost Sect Reggio Emilia, Reggio Emilia, Italy

[3] Anim Hlth Res Ctr, Madrid, Spain

[4] Univ Vet Med, Dept Infect Dis, Inst Virol, Hannover, Germany

[5] Vet & Agrochem Res Ctr, Uccle, Belgium

[6] Natl Vet Inst, S-75007 Uppsala, Sweden

[7] Inst Virol & Immunoprophylaxis, Mittelhausern, Switzerland

[8] French Agcy Food Environm & Occupat Hlth Safety, Swine Virol & Immunol Sect, Ploufragan, France

[9] Cent Vet Inst, Lelystad, Netherlands

[10] Tech Univ Denmark, Natl Vet Inst, Kalvehave, Denmark

[11] Natl Inst Vet Res, Dept Swine Dis, Pulawy, Poland

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2011年 / 23卷 / 05期

关键词：

Classical swine fever virus; polymerase chain reaction; ring trial; RT-PCR ASSAY; PESTIVIRUSES; VALIDATION; INFECTION; VIRULENCE; VACCINE; TAQMAN; PROBES; SIGNS; PIGS;

D O I：

10.1177/1040638711416849

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The current study reports on a real-time reverse transcription polymerase chain reaction (real-time RT-PCR) ring trial for the detection of Classical swine fever virus (CSFV) genomic RNA undertaken by 10 European laboratories. All laboratories were asked to use their routine in-house real-time RT-PCR protocols and a standardized protocol commonly used by the Friedrich-Loeffler-Institute (FLI) on a panel of well-characterized samples. In general, all participants produced results within the acceptable range. The FLI assay, several in-house assays, and the commercial kits had high analytical sensitivity and specificity values. Nevertheless, some in-house systems had unspecific reactions or suboptimal sensitivity with only a single CSFV genotype. Follow-up actions involved either improvement of suboptimal assays or replacement of specific laboratory assays with the FLI protocol, with or without modifications. In conclusion, the ring trial showed reliability of classical swine fever diagnosis on an international level and helped to optimize CSFV-specific RT-PCR diagnostics.

引用

页码：999 / 1004

页数：6

共 24 条

[1] Simultaneous detection of Classical swine fever virus and North American genotype Porcine reproductive and respiratory syndrome virus using a duplex real-time RT-PCR [J].