Expression, renaturation and functional analysis of an excitatory insect-specific toxin from scorpion Buthus martensii Karsch

被引：10
|
作者：
Li, C
Liu, W
Bossmans, F
Zhu, RH
Tytgat, J
Wang, DC [1 ]
机构：
[1] Chinese Acad Sci, Inst Biophys, Ctr Struct & Mol Biol, Beijing 100101, Peoples R China
[2] Univ Louvain, Toxicol Lab, B-3000 Louvain, Belgium
来源：
PROTEIN AND PEPTIDE LETTERS | 2005年 / 12卷 / 07期
关键词：
BmK IT-AP; E; coli; recombinant expression; denaturation and renaturation;
D O I：
10.2174/0929866054696172
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The cDNA of BmK IT-AP, an excitatory insect toxin from the scorpion Buthus martensi Karsch that has an analgesic effect on mammalian cells, was expressed in E. coli in the form of an inclusion body. Following denaturation and reduction, the recombinant protein was renatured and purified by liquid chromatography. The authenticity of the recombinant product was confirmed by bioassay and its electrophysiological effect on insect sodium channel.
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收藏
页码：635 / 638
页数：4
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