Optimization of the determination of inorganic and organic selenium species using high-performance liquid chromatography - Electrothermal atomic absorption spectrometry

被引:60
作者
Gilon, N
PotinGautier, M
Astruc, M
机构
关键词
selenite; selenate; selenocystine; selenomethionine; seleno amino acids;
D O I
10.1016/0021-9673(96)00484-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The liquid chromatographic simultaneous separation of selenite, selenate, selenocystine and selenomethionine was studied with a selenium-specific detector; electrothermal atomic absorption spectrometry. Three chromatographic modes were compared; two ion-pairing ones using either a cationic or an anionic reagent and ion-exchange. Separation parameters such as composition, pH and concentration of the mobile phase were investigated. The influence of chromatographic conditions on the sensitivity of the detector is studied. The ion-exchange method was determined to give the best results, with detection limits ranging from 8 to 17 mu g l(-1) within a 30 min separation time. An application of this method to the analysis of seleno compounds present in a selenium-rich yeast after enzymic hydrolysis extraction is presented.
引用
收藏
页码:327 / 334
页数:8
相关论文
共 21 条
[1]   CHEMICAL FORMS OF SELENIUM IN RAT-TISSUES AFTER ADMINISTRATION OF SELENITE OR SELENOMETHIONINE [J].
BEILSTEIN, MA ;
WHANGER, PD .
JOURNAL OF NUTRITION, 1986, 116 (09) :1711-1719
[2]   DIRECT DETERMINATION OF SELENO-AMINO ACIDS IN BIOLOGICAL TISSUES BY ANION-EXCHANGE SEPARATION AND ELECTROCHEMICAL DETECTION [J].
CAVALLI, S ;
CARDELLICCHIO, N .
JOURNAL OF CHROMATOGRAPHY A, 1995, 706 (1-2) :429-436
[3]   HITACHI ZEEMAN GRAPHITE-FURNACE ATOMIC-ABSORPTION SPECTROMETER AS A SELENIUM-SPECIFIC DETECTOR FOR ION CHROMATOGRAPHY - SEPARATION AND DETERMINATION OF SELENITE AND SELENATE [J].
CHAKRABORTI, D ;
HILLMAN, DCJ ;
IRGOLIC, KJ ;
ZINGARO, RA .
JOURNAL OF CHROMATOGRAPHY, 1982, 249 (01) :81-92
[4]   ANALYTICAL METHODS FOR THE SPECIATION OF SELENIUM-COMPOUNDS - A REVIEW [J].
DAUCHY, X ;
POTINGAUTIER, M ;
ASTRUC, A ;
ASTRUC, M .
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1994, 348 (12) :792-805
[5]  
DUBOIS F, 1988, PATHOL BIOL, V38, P1017
[6]   DETERMINATION OF SELENIUM BY GAS-CHROMATOGRAPHY ELECTRON-CAPTURE DETECTION USING A RAPID DERIVATIZATION PROCEDURE [J].
ELASEER, A ;
NICKLESS, G .
JOURNAL OF CHROMATOGRAPHY A, 1994, 664 (01) :77-87
[7]   FLOW-INJECTION AND CONTINUOUS-FLOW SYSTEMS FOR THE DETERMINATION OF SE(IV) AND SE(VI) BY HYDRIDE GENERATION ATOMIC-ABSORPTION SPECTROMETRY WITH ONLINE PREREDUCTION OF SE(VI) TO SE(IV) [J].
FERNANDEZ, MGC ;
PALACIOS, MA ;
CAMARA, C .
ANALYTICA CHIMICA ACTA, 1993, 283 (01) :386-392
[8]   BIOAVAILABILITY PREDICTION OF AMINO-ACIDS AND PEPTIDES IN NUTRITIVE MIXTURES BY SEPARATION ON NONPOLAR STATIONARY PHASES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND DIRECT UV DETECTION AT 210 NM [J].
FIALAIRE, A ;
POSTAIRE, E ;
PROGNON, R ;
PRADEAU, D .
JOURNAL OF LIQUID CHROMATOGRAPHY, 1993, 16 (14) :3003-3015
[9]  
GAUTIER MP, 1993, APPL ORGANOMET CHEM, V7, P593
[10]   SELENOAMINO ACID SPECIATION USING HPLC-ETAAS FOLLOWING AN ENZYMATIC-HYDROLYSIS OF SELENOPROTEIN [J].
GILON, N ;
ASTRUC, A ;
ASTRUC, M ;
POTINGAUTIER, M .
APPLIED ORGANOMETALLIC CHEMISTRY, 1995, 9 (07) :623-628