Omega-3 polyunsaturated fatty acids alleviate hepatic steatosis-induced inflammation through Sirt1-mediated nuclear translocation of NF-κB p65 subunit in hepatocytes of large yellow croaker (Larmichthys crocea)

Hepatic steatosis induced inflammation is becoming increasingly prevalent in farmed fish. This study was conducted to investigate the protective effects of omega-3 polyunsaturated fatty acids omega-3 PUFAs) against hepatic steatosis-induced inflammation and its potential molecular mechanisms in hepatocyte of large yellow croaker (Larmichthys crocea). We found that the hepatic steatosis-induced inflammation was relieved by omega-3 PUFAs, meanwhile, the Sirt1 activity and transcript expression was increased by omega-3 PUFAs. The increased Sirtl activity can decrease the hepatic steatosis-induced inflammation. The protective effects of omega-3 PUFAs against hepatic steatosis-induced inflammation was reversed by the treatment with Sirtl inhibitor EX-527. The nuclear translocation of nuclear transcription factor kappa-B (NF-kappa B) p65 was significantly decreased after omega-3 PUFAs treatments compared to the palmitic acid stimulation group. The omega-3 PUFAs induced cytoplasm translocation of NF-kappa B p65 was reversed by EX-527. Together, omega-3 PUFAs alleviate hepatic steatosis-induced inflammation through Sirtl-mediated nuclear translocation of NF-kappa B p65 subunit in hepatocytes of large yellow croaker. The present study provides important insight into the mechanisms of the protective effects of omega-3 PUFAs, providing theory bases for alleviating the hepatic steatosis induced inflammation of farmed fish, thereby offering great benefits to the aquaculture industry and fish consumers. (C) 2017 Published by Elsevier Ltd.