RECORDING NEURONAL ACTIVITY ON CHIP WITH SEGMENTED 3D MICROELECTRODE ARRAYS

被引：0

作者：

Revyn, Nele ^{[1
]}

Hu, Michel H. Y. ^{[2
]}

Frimat, Jean-Philippe M. S. ^{[2
]}

De Wagenaar, Bjorn ^{[1
]}

Van den Maagdenberg, Arn M. J. M. ^{[2
]}

Sarro, Pasqualina M. ^{[1
]}

Mastrangeli, Massimo ^{[1
]}

机构：

[1] Delft Univ Technol, Dept Microelect, ECTM, Delft, Netherlands

[2] Leiden Univ, Human Genet & Neurol, Med Ctr, Leiden, Netherlands

来源：

2022 IEEE 35TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS CONFERENCE (MEMS) | 2022年

基金：

欧盟地平线“2020”;

关键词：

brain-on-chip; electrodes; electrophysiology; microelectrode array; microfabrication; neurons; organ-on-chip;

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We present preliminary recordings on chip of three-dimensional (3D) electric neuronal activity from cultures of cortical neurons derived from human-induced pluripotent stem cells (hiPSCs). The recordings were obtained through 3D microelectrode arrays (MEAs) composed of truncated, 90 mu m-high Si micropyramids endowed with multiple, electrically distinct, and vertically arranged TiN microelectrodes. The unique design and implementation of the 3D microelectrodes, complemented by a 60-electrode readout interface, allow for 3D spatial recording of neuronal activity, as well as single-unit recordings in high throughput, which are currently not possible with commercial MEA platforms. Future work will aim at optimizing extended 3D MEAs over optically transparent substrates for electro-physiological investigation of 3D neuronal tissues and organoids.

引用

页码：102 / 105

页数：4

共 15 条

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