Effect of genotype and medium culture content on microspore-derived embryo formation in Chinese cabbage (Brassica rapa ssp chinensis) cv. Lastochka

被引：24

作者：

Shumilina, D. V. ^{[1
]}

Shmykova, N. A. ^{[1
]}

Bondareva, L. L. ^{[1
]}

Suprunova, T. P. ^{[1
]}

机构：

[1] All Russia Res Inst Vegetable Breeding & Seed Pro, P VNIISSOK, Odintsovo Dist 143080, Moscow Region, Russia

来源：

BIOLOGY BULLETIN | 2015年 / 42卷 / 04期

关键词：

HIGH-FREQUENCY EMBRYOGENESIS; PLANT-REGENERATION; ACTIVATED-CHARCOAL; ANTHER CULTURE; OILSEED RAPE; NAPUS L; CAMPESTRIS; GROWTH; BRASSINOLIDE; DESICCATION;

D O I：

10.1134/S1062359015040135

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The influence of different factors on microspore embryogenesis in Chinese cabbage (B. rapa ssp. chinensis) was studied. A genotype dependence for embryo formation was observed. The majority of embryos and plants were obtained from microspores isolated from flower buds (2-2.9 mm in length) and cultured in the NLN liquid medium with 13% sucrose (w/v) supplemented with 24-epibrassinolide and 1% activated charcoal. Embryos cultured on the 1/2 Murashige-Skoog culture medium with 2% sucrose (w/v), 0.1 mg/L benzylaminopurine, and 3 g/L Phytagel stimulated the formation of secondary embryos that resulted in development of large number of doubled haploid plants.

引用

页码：302 / 309

页数：8

共 50 条

[1] DIFFERENTIAL STAINING OF ABORTED AND NONABORTED POLLEN [J].