Artificial lantipeptides from in vitro translations

被引：34

作者：

Seebeck, Florian P. ^{[1
,2
,3
]}

Ricardo, Alonso ^{[1
,2
,4
]}

Szostak, Jack W. ^{[1
,2
]}

机构：

[1] Massachusetts Gen Hosp, Howard Hughes Med Inst, Boston, MA 02114 USA

[2] Massachusetts Gen Hosp, Ctr Computat & Integrat Biol, Simches Res Ctr, Boston, MA 02114 USA

[3] Max Planck Inst Mol Physiol, Dept Phys Biochem, D-44139 Dortmund, Germany

[4] Ra Pharmaceut Inc, Boston, MA USA

来源：

CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 21期

基金：

美国国家卫生研究院;

关键词：

DEHYDROALANINE; PEPTIDES; BIOSYNTHESIS; CONVERSION; MECHANISM; ANALOGS; NISIN;

D O I：

10.1039/c0cc05663d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have devised a protocol for enzyme-free insertion of dehydroalanine, dehydrobutyrine and thioether crosslinks into translated peptides. In vitro translation using 4-selenalysine and 4-selenoisoleucine as substitutes for lysine and isoleucine yields peptides that can be converted to polycyclic structures using mild chemistry in water. This methodology presents a gateway for exploring the potential of artificial lantipeptides as scaffolds for drug development.

引用

页码：6141 / 6143

页数：3

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