CRISPR/Cas system for yeast genome engineering: advances and applications

被引:127
作者
Stovicek, Vratislav [1 ]
Holkenbrink, Carina [1 ]
Borodina, Irina [1 ]
机构
[1] Tech Univ Denmark, Novo Nordisk Fdn Ctr Biosustainabil, Kemitorvet 220, DK-2800 Lyngby, Denmark
基金
欧盟第七框架计划;
关键词
CRISPR/Cas; CRISPR interference; genome editing; yeasts; CRISPR transcriptional regulation; Saccharomyces cerevisiae; SACCHAROMYCES-CEREVISIAE; GUIDE RNA; CHROMOSOMAL INTEGRATION; DESIGN; TOOL; MARKER; GENES; CAS9; WEB; TRANSCRIPTION;
D O I
10.1093/femsyr/fox030
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The methods based on the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system have quickly gained popularity for genome editing and transcriptional regulation in many organisms, including yeast. This review aims to provide a comprehensive overview of CRISPR application for different yeast species: from basic principles and genetic design to applications.
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页数:16
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