Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil

被引:87
作者
Bueris, Vanessa
Sircili, Marcelo Palma
Taddei, Carla Romano
dos Santos, Maurilio Fernandes
Franzolin, Marcia Regina
Martinez, Marina Aquerizo [1 ]
Ferrer, Suzana Ramos [2 ]
Barreto, Mauricio Lima [2 ]
Trabulsi, Luiz Rachid
机构
[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Anal Clin & Toxicol, BR-05508 Sao Paulo, Brazil
[2] Univ Fed Bahia, Inst Saude Coletiva, Salvador, BA, Brazil
来源
MEMORIAS DO INSTITUTO OSWALDO CRUZ | 2007年 / 102卷 / 07期
关键词
diarrheagenic Escherichia coli; virulence; multiplex PCR;
D O I
10.1590/S0074-02762007005000116
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
We identified different diarrheagenic (DEC) Escherichia coli pathotypes isolated from 1,207 children with and without acute endemic diarrhea in Salvador Bahia, Brazil collected as part of a case-control study. Since the identification of DEC cannot be based on only biochemical and culture criteria, we used a multiplex polymerase chain reaction developed by combining five specific primer pairs for Enteropathogenic Escherichia coli (EPEC), Shiga toxin-producing E. coli/Enterohaemorrhagic E. coli (STEC/EHEC), Enterotoxigenic E. coli (ETEQ and Enteroaggregative E. coli (EAEC) to detect these pathotypes simultaneously in a single-step reaction. In order to distinguish typical and atypical EPEC strains, these were tested for the presence of EAF plasmid. The prevalence of diarrheagenic E. coli in this sample of a global case-control study was 25.4% (259 patients) and 18.7% (35 patients) in the diarrhea group (1, 020 patients) and the control group (18 7 patients), respectively. The most frequently isolated pathotype was EAEC (10.7%), followed by atypical EPEC (9.4%), ETEC (3.7%), and STEC (0.6%). Typical EPEC was detected only in one sample. The prevalence of the pathotypes studied in children with diarrhea was not significantly different from that in children without diarrhea.
引用
收藏
页码:839 / 844
页数:6
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