Assessment of shikonin for potential estrogenic activity by dual-luciferase reporter based bioluminescent measurements in vitro

被引：2

作者：

Zhang, Yi ^{[2
,3
]}

Li, Ping-Ping ^{[1
]}

机构：

[1] Peking Univ, Dept Integrated Tradit Chinese & Western Med, Key Lab Carcinogenesis & Translat Res,Minist Educ, Sch Oncol,Beijing Canc Hosp & Inst, Beijing 100142, Peoples R China

[2] Beijing Univ Chinese Med, Dept Anat, Sch Preclin Med, Beijing, Peoples R China

[3] Peking Univ, Dept Integrated Tradit Chinese & Western Med, Sch Basic Med Sci, Beijing 100142, Peoples R China

来源：

PHARMAZIE | 2011年 / 66卷 / 02期

关键词：

STEROID SULFATASE GENES; LITHOSPERMUM-ERYTHRORHIZON; CELLS; PHARMACOLOGY; ACTIVATION; MECHANISMS; EXPRESSION; EXTRACT; CANCER;

D O I：

10.1691/ph.2011.0714

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Shikonin, an active component of Lithospermum erythrorhizon Sieb. et Zucc., shows multiple pharmacological properties. However, the estrogenic activity of shikonin is remaining unclear. We assessed the potential estrogenic activity of shikonin with dual-luciferase reporter assay and bioluminescent measurements, by using transient cotransfection with estrogen dependent plasmid pERE-TK-Luc and internal control plasmid pRL-TK in MCF-7 cells. Estrogenic activity of shikonin, even at high concentration did not alter significantly compared to negative control (p > 0.05) and were significantly lower than those with E-2 (p < 0.01). Concluding, shikonin demonstrates no estrogenic activity in vitro.

引用

页码：141 / 143

页数：3

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