Human bone marrow mesenchymal stem cells for retinal vascular injury

被引:23
作者
Wang, Jin-Da [1 ]
An, Ying [1 ]
Zhang, Jing-Shang [1 ]
Wan, Xiu-Hua [1 ]
Jonas, Jost B. [1 ,2 ]
Xu, Liang [1 ]
Zhang, Wei [1 ]
机构
[1] Capital Med Univ, Beijing Tongren Hosp, Beijing Tongren Eye Ctr, Beijing Inst Ophthalmol,Beijing Key Lab Ophthalmo, Beijing, Peoples R China
[2] Heidelberg Univ, Med Fac Mannheim, Dept Ophthalmol, Seegartenklin, Heidelberg, Germany
关键词
blood-retina barrier; bone marrow mesenchymal cells; diabetic retinopathy; neuroprotection; neuroregeneration; retinal protection; retinal regeneration; retinal vascular injury; retinopathy of prematurity; INTRAVITREAL TRIAMCINOLONE ACETONIDE; DIABETIC MACULAR EDEMA; RETINITIS-PIGMENTOSA; STROMAL CELLS; DEGENERATION; TRANSPLANTATION; RETINOPATHY; VISION; QUANTIFICATION; ANGIOGENESIS;
D O I
10.1111/aos.13154
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
PurposeTo examine the potential of intravitreally implanted human bone marrow-derived mesenchymal stem cells (BMSCs) to affect vascular repair and the blood-retina barrier in mice and rats with oxygen-induced retinopathy, diabetic retinopathy or retinal ischaemia-reperfusion damage. MethodsThree study groups (oxygen-induced retinopathy group: 18 C57BL/6J mice; diabetic retinopathy group: 15 rats; retinal ischaemia-reperfusion model: 18 rats) received BMSCs injected intravitreally. Control groups (oxygen-induced retinopathy group: 12 C57BL/6J mice; diabetic retinopathy group: 15 rats; retinal ischaemia-reperfusion model: 18 rats) received an intravitreal injection of phosphate-buffered saline. We applied immunohistological techniques to measure retinal vascularization, spectroscopic measurements of intraretinally extravasated fluorescein-conjugated dextran to quantify the blood-retina barrier breakdown, and histomorphometry to assess retinal thickness and retinal ganglion cell count. ResultsIn the oxygen-induced retinopathy model, the study group with intravitreally injected BMSCs as compared with the control group showed a significantly (p=0.001) smaller area of retinal neovascularization. In the diabetic retinopathy model, study group and control group did not differ significantly in the amount of intraretinally extravasated dextran. In the retinal ischaemia-reperfusion model, on the 7th day after retina injury, the retina was significantly thicker in the study group than in the control group (p=0.02), with no significant difference in the retinal ganglion cell count (p=0.36). ConclusionsIntravitreally implanted human BMSCs were associated with a reduced retinal neovascularization in the oxygen-induced retinopathy model and with a potentially cell preserving effect in the retinal ischaemia-reperfusion model. Intravitreal BMSCs may be of potential interest for the therapy of retinal vascular disorders.
引用
收藏
页码:E453 / E461
页数:9
相关论文
共 33 条
[11]   Predictive factors for visual acuity after intravitreal triamcinolone treatment for diabetic macular edema [J].
Jonas, JB ;
Martus, P ;
Degenring, RF ;
Kreissig, I ;
Akkoyun, I .
ARCHIVES OF OPHTHALMOLOGY, 2005, 123 (10) :1338-1343
[12]   Diffuse diabetic macular oedema treated by intravitreal triamcinolone acetonide: a comparative, non-randomised study [J].
Jonas, JB ;
Akkoyun, I ;
Kreissig, I ;
Degenring, RF .
BRITISH JOURNAL OF OPHTHALMOLOGY, 2005, 89 (03) :321-326
[13]   Exudative age-related macular degeneration treated by intravitreal triamcinolone acetonide. A prospective comparative nonrandomized study [J].
Jonas, JB ;
Degenring, RF ;
Kreissig, I ;
Friedemann, T ;
Akkoyun, I .
EYE, 2005, 19 (02) :163-170
[14]   Angiogenesis-independent endothelial protection of liver: Role of VEGFR-1 [J].
LeCouter, J ;
Moritz, DR ;
Li, B ;
Phillips, GL ;
Liang, XH ;
Gerber, HP ;
Hillan, KJ ;
Ferrara, N .
SCIENCE, 2003, 299 (5608) :890-893
[15]   Intravitreal Injections of Neurotrophic Factors Secreting Mesenchymal Stem Cells Are Neuroprotective in Rat Eyes following Optic Nerve Transection [J].
Levkovitch-Verbin, Hani ;
Sadan, Ofer ;
Vander, Shelly ;
Rosner, Mordechai ;
Barhum, Yael ;
Melamed, Eldad ;
Offen, Daniel ;
Melamed, Shlomo .
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2010, 51 (12) :6394-6400
[16]   Effects of bone-marrow mesenchymal stem cells transplanted into vitreous cavity of rat injured by ischemia/reperfusion [J].
Li Na ;
Li Xiao-rong ;
Yuan Jia-qin .
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY, 2009, 247 (04) :503-514
[17]   Liver organogenesis promoted by endothelial cells prior to vascular function [J].
Matsumoto, K ;
Yoshitomi, H ;
Rossant, J ;
Zaret, KS .
SCIENCE, 2001, 294 (5542) :559-563
[18]   Rescue of retinal degeneration by intravitreally injected adult bone marrow-derived lineage-negative hematopoietic stem cells [J].
Otani, A ;
Dorrell, MI ;
Kinder, K ;
Moreno, SK ;
Nusinowitz, S ;
Banin, E ;
Heckenlively, J ;
Friedlander, M .
JOURNAL OF CLINICAL INVESTIGATION, 2004, 114 (06) :765-774
[19]   Myeloid progenitors differentiate into microglia and promote vascular repair in a model of ischemic retinopathy [J].
Ritter, Matthew R. ;
Banin, Eyal ;
Moreno, Stacey K. ;
Aguilar, Edith ;
Dorrell, Michael I. ;
Friedlander, Martin .
JOURNAL OF CLINICAL INVESTIGATION, 2006, 116 (12) :3266-3276
[20]  
Rogers S, 2010, OPHTHALMOLOGY, V117, P313, DOI [10.1016/j.ophtha.2009.07.017, 10.1016/j.ophtha.2010.01.060]