SsrA (tmRNA) acts as an antisense RNA to regulate Staphylococcus aureus pigment synthesis by base pairing with crtMN mRNA

被引：28

作者：

Liu, Yu ^{[1
]}

Wu, Na ^{[1
]}

Dong, Jie ^{[1
]}

Gao, Yaping ^{[1
]}

Zhang, Xin ^{[1
]}

Shao, Ningsheng ^{[1
]}

Yang, Guang ^{[1
]}

机构：

[1] Beijing Inst Basic Med Sci, Beijing 100850, Peoples R China

来源：

FEBS LETTERS | 2010年 / 584卷 / 20期

基金：

中国国家自然科学基金;

关键词：

Staphylococcus aureus; Pigment; ssrA; crtMN; ESCHERICHIA-COLI; VIRULENCE FACTORS; RIBOSOME RESCUE; NONCODING RNAS; 10SA RNA; PROTEIN; TRANSLATION; EXPRESSION; MOLECULE; BACTERIA;

D O I：

10.1016/j.febslet.2010.09.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SsrA RNA (small stable RNA A), also known as tmRNA and 10Sa RNA, functions both as tRNA and mRNA through its unique structure. The carotenoid pigment is the eponymous feature of human pathogen Staphylococcus aureus. Here, we found that the pigment of the mutant strain with ssrA deletion was increased. Furthermore, it was demonstrated that ssrA could act as an antisense RNA aside from its well-known biological function, and crtMN, encoding two essential enzymes for the pigment synthesis, was identified as target of ssrA. Further investigation showed ssrA could specifically base pair with the RBS (ribosomal binding site) region of the crtMN mRNA. Our results revealed a new mechanism by which ssrA regulated the biosynthesis of pigment in S. aureus. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4325 / 4329

页数：5

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