New trends in fluorescence in situ hybridization for identification and functional analyses of microbes

被引：67
作者：
Wagner, Michael [1 ]
Haider, Susanne [1 ]
机构：
[1] Univ Vienna, Dept Microbial Ecol, A-1090 Vienna, Austria
来源：
CURRENT OPINION IN BIOTECHNOLOGY | 2012年 / 23卷 / 01期
关键词：
CATALYZED REPORTER DEPOSITION; ROLLING-CIRCLE AMPLIFICATION; BACTERIAL-CELLS; RIBOSOMAL-RNA; OLIGONUCLEOTIDE PROBES; POLYNUCLEOTIDE PROBES; STAINING TECHNIQUES; CLICK CHEMISTRY; MICROORGANISMS; FISH;
D O I：
10.1016/j.copbio.2011.10.010
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Fluorescence in situ hybridization (FISH) has become an indispensable tool for rapid and direct single-cell identification of microbes by detecting signature regions in their rRNA molecules. Recent advances in this field include new web-based tools for assisting probe design and optimization of experimental conditions, easy-to-implement signal amplification strategies, innovative multiplexing approaches, and the combination of FISH with transmission electron microscopy or extracellular staining techniques. Further emerging developments focus on sorting FISH-identified cells for subsequent single-cell genomics and on the direct detection of specific genes within single microbial cells by advanced FISH techniques employing various strategies for massive signal amplification.
引用
收藏
页码：96 / 102
页数：7
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