Purification and characterization of a glucan from Bacillus Calmette Guerin and the antitumor activity of its sulfated derivative

被引：18
作者：
Xu, Xiongbo [1 ,2 ]
Gu, Zixin [3 ]
Liu, Shao [1 ,2 ]
Gao, Na [4 ]
He, Xiaozhen [5 ]
Xin, Xiu [3 ]
机构：
[1] Xiangya Hosp, Dept Pharm, Changsha 410008, Hunan, Peoples R China
[2] Cent S Univ, Sch Pharm, Changsha 410013, Hunan, Peoples R China
[3] Jiuzhitang Co Ltd, Changsha 410021, Hunan, Peoples R China
[4] Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China
[5] Cent S Univ, Tumor Inst, Changsha 410078, Hunan, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2015年 / 128卷
关键词：
BCG-PSN; Purification; Characterization; Sulfated derivative; Antitumor; ANTIOXIDANT ACTIVITY; IMMUNOSTIMULATORY ACTIVITY; STRUCTURAL FEATURES; POLYSACCHARIDES; ANTIGLYCATION; AMYGDALIN;
D O I：
10.1016/j.carbpol.2015.04.025
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A water-soluble glucan, BCG-PASW, with a molecular weight of 2.10 x 10(4) Da, was separated from polysaccharide nucleic acid fraction of Bacillus Calmette Guerin (BCG-PSN) using DEAE-52 cellulose and Sephadex G-200 chromatography. Based on gas chromatography-mass spectrometry (GC-MS), fourier transform infrared (FT-IR) spectra, 1D and 2D nuclear magnetic resonance (NMR) spectroscopy techniques (COSY, HSQC and HMBC), BCG-PASW was found to be an alpha-D-glucan composed of alpha-D-(1 -> 4)-linked glucopyranosyl residues, with branches at O-6 consisting of non-reducing terminal alpha-D-Glcp approximately every eight residues. In vitro antitumor activity by MTS method, its sulfated derivative with a substitution degree of 0.59, could inhibite C666-1 nasopharyngeal carcinoma cells growth significantly. The results indicated that the sulfate content play a decisive role in the bioactivities of the polysaccharides. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：138 / 146
页数：9
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