UV irradiation inhibits ABC transporters via generation of ADP-ribose by concerted action of poly(ADP-ribose) polymerase-1 and glycohydrolase

被引：26

作者：

Dumitriu, IE ^{[1
]}

Voll, RE ^{[1
]}

Kolowos, W ^{[1
]}

Gaipl, US ^{[1
]}

Heyder, P ^{[1
]}

Kalden, JR ^{[1
]}

Herrmann, M ^{[1
]}

机构：

[1] Univ Erlangen Nurnberg, Inst Clin Immunol, Dept Internal Med 3, D-91054 Erlangen, Germany

来源：

CELL DEATH AND DIFFERENTIATION | 2004年 / 11卷 / 03期

关键词：

ABC transporters; PARP-1; PARP-null cells; PARG;

D O I：

10.1038/sj.cdd.4401348

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

ATP-binding cassette (ABC) transporters are involved in the transport of multiple substrates across cellular membranes, including metabolites, proteins, and drugs. Employing a functional fluorochrome export assay, we found that UVB irradiation strongly inhibits the activity of ABC transporters. Specific inhibitors of poly(ADP-ribose) polymerase-1 (PARP-1) restored the function of ABC transporters in UVB-irradiated cells, and PARP-1-deficient cells did not undergo UVB-induced membrane transport inhibition. These data suggest that PARP-1 activation is necessary for ABC transporter functional downregulation. The hydrolysis of poly(ADP-ribose) by poly(ADP-ribose) glycohydrolase (PARG) was also required, since specific PARG inhibitors, which limit the production of ADP-ribose molecules, restored the function of ABC transporters. Furthermore, ADP-ribose molecules potently inhibited the activity of the ABC transporter P-glycoprotein. Hence, poly(ADP-ribose) metabolism appears to play a novel role in the regulation of ABC transporters.

引用

页码：314 / 320

页数：7

共 41 条

[1] POLY(ADP-RIBOSE) CATABOLISM IN MAMMALIAN-CELLS EXPOSED TO DNA-DAMAGING AGENTS [J].

ALVAREZGONZALEZ, R ;

ALTHAUS, FR .

MUTATION RESEARCH, 1989, 218 (02) :67-74

[2] The secretory route of the leaderless protein interleukin 1β involves exocytosis of endolysosome-related vesicles [J].

Andrei, C ;

Dazzi, C ;

Lotti, L ;

Torrisi, MR ;

Chimini, G ;

Rubartelli, A .

MOLECULAR BIOLOGY OF THE CELL, 1999, 10 (05) :1463-1475

[3] Inhibition of nitric oxide synthase ameliorates cellular injury in sickle cell mouse kidneys [J].

Bank, N ;

Kiroycheva, M ;

Singhal, PC ;

Anthony, GM ;

Southan, GJ ;

Szabo, C .

KIDNEY INTERNATIONAL, 2000, 58 (01) :82-89

[4] Comparative effects of Bcl-2 over-expression and ZVAD.FMK treatment on dexamethasone and VP16-induced apoptosis in CEM cells [J].

Benson, RSP ;

Dive, C ;

Watson, AJM .

CELL DEATH AND DIFFERENTIATION, 1998, 5 (05) :432-439

[5] CXCR4 and CD4 mediate a rapid CD95-independent cell death in CD4+ T cells [J].

Berndt, C ;

Möpps, B ;

Angermüller, S ;

Gierschik, P ;

Krammer, PH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (21) :12556-12561

[6] Specific docking of apolipoprotein A-I at the cell surface requires a functional ABCA1 transporter [J].

Chambenoit, O ;

Hamon, Y ;

Marguet, D ;

Rigneault, H ;

Rosseneu, M ;

Chimini, G .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (13) :9955-9960

[7] INTERNAL DUPLICATION AND HOMOLOGY WITH BACTERIAL TRANSPORT PROTEINS IN THE MDR1 (P-GLYCOPROTEIN) GENE FROM MULTIDRUG-RESISTANT HUMAN-CELLS [J].

CHEN, CJ ;

CHIN, JE ;

UEDA, K ;

CLARK, DP ;

PASTAN, I ;

GOTTESMAN, MM ;

RONINSON, IB .

CELL, 1986, 47 (03) :381-389

[8] OVEREXPRESSION OF A TRANSPORTER GENE IN A MULTIDRUG-RESISTANT HUMAN LUNG-CANCER CELL-LINE [J].

COLE, SPC ;

BHARDWAJ, G ;

GERLACH, JH ;

MACKIE, JE ;

GRANT, CE ;

ALMQUIST, KC ;

STEWART, AJ ;

KURZ, EU ;

DUNCAN, AMV ;

DEELEY, RG .

SCIENCE, 1992, 258 (5088) :1650-1654

[9] Importance of poly(ADP-ribose) glycohydrolase in the control of poly(ADP-ribose) metabolism [J].

Davidovic, L ;

Vodenicharov, M ;

Affar, EB ;

Poirier, GG .

EXPERIMENTAL CELL RESEARCH, 2001, 268 (01) :7-13

[10]

Dean M, 2001, J LIPID RES, V42, P1007

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