Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster

被引:23
作者
Gonzales, Cesar Nava [1 ]
McKaughan, Quintyn [1 ]
Bushong, Eric A. [2 ]
Cauwenberghs, Kalyani [1 ]
Ng, Renny [1 ]
Madany, Matthew [2 ]
Ellisman, Mark H. [2 ]
Su, Chih-Ying [1 ]
机构
[1] Univ Calif San Diego, Div Biol Sci, Neurobiol Sect, San Diego, CA 92103 USA
[2] Univ Calif San Diego, Natl Ctr Microscopy & Imaging Res, Ctr Res Biol Syst, San Diego, CA 92103 USA
来源
ELIFE | 2021年 / 10卷
基金
美国国家科学基金会;
关键词
CHEMOSENSORY RECEPTORS; BINDING-PROTEINS; MOLECULAR-BASIS; ORGANIZATION; ARCHITECTURE; INHIBITION; RESPONSES; ORGANS; ATLAS;
D O I
10.7554/eLife.69896
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The biophysical properties of sensory neurons are influenced by their morphometric and morphological features, whose precise measurements require high-quality volume electron microscopy (EM). However, systematic surveys of nanoscale characteristics for identified neurons are scarce. Here, we characterize the morphology of Drosophila olfactory receptor neurons (ORNs) across the majority of genetically identified sensory hairs. By analyzing serial block-face electron microscopy images of cryofixed antennal tissues, we compile an extensive morphometric data set based on 122 reconstructed 3D models for 33 of the 40 identified antennal ORN types. Additionally, we observe multiple novel features-including extracellular vacuoles within sensillum lumen, intricate dendritic branching, mitochondria enrichment in select ORNs, novel sensillum types, and empty sensilla containing no neurons-which raise new questions pertinent to cell biology and sensory neurobiology. Our systematic survey is critical for future investigations into how the size and shape of sensory neurons influence their responses, sensitivity, and circuit function.
引用
收藏
页数:30
相关论文
共 41 条
[1]   Variant Ionotropic Glutamate Receptors as Chemosensory Receptors in Drosophila [J].
Benton, Richard ;
Vannice, Kirsten S. ;
Gomez-Diaz, Carolina ;
Vosshall, Leslie B. .
CELL, 2009, 136 (01) :149-162
[2]   Molecular, anatomical, and functional organization of the Drosophila olfactory system [J].
Couto, A ;
Alenius, M ;
Dickson, BJ .
CURRENT BIOLOGY, 2005, 15 (17) :1535-1547
[3]   Odor coding in the Drosophila antenna [J].
de Bruyne, M ;
Foster, K ;
Carlson, JR .
NEURON, 2001, 30 (02) :537-552
[4]   Integrating the molecular and cellular basis of odor coding in the Drosophila antenna [J].
Dobritsa, AA ;
van der Goes van Naters, W ;
Warr, CG ;
Steinbrecht, RA ;
Carlson, JR .
NEURON, 2003, 37 (05) :827-841
[5]   Pheromones mediating copulation and attraction in Drosophila [J].
Dweck, Hany K. M. ;
Ebrahim, Shimaa A. M. ;
Thoma, Michael ;
Mohamed, Ahmed A. M. ;
Keesey, Ian W. ;
Trona, Federica ;
Lavista-Llanos, Sofia ;
Svatos, Ales ;
Sachse, Silke ;
Knaden, Markus ;
Hansson, Bill S. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2015, 112 (21) :E2829-E2835
[6]   Olfactory Preference for Egg Laying on Citrus Substrates in Drosophila [J].
Dweck, Hany K. M. ;
Ebrahim, Shimaa A. M. ;
Kromann, Sophie ;
Bown, Deni ;
Hillbur, Ylva ;
Sachse, Silke ;
Hansson, Bill S. ;
Stensmyr, Marcus C. .
CURRENT BIOLOGY, 2013, 23 (24) :2472-2480
[7]  
Fernandez I, 2020, BIORXIV PREPRINT, DOI [10.1101/2020.11.08.371096, DOI 10.1101/2020.11.08.371096]
[8]   Genetic and functional subdivision of the Drosophila antennal lobe [J].
Fishilevich, E ;
Vosshall, LB .
CURRENT BIOLOGY, 2005, 15 (17) :1548-1553
[9]   Mitochondrial Ca2+ mobilization is a key element in olfactory signaling [J].
Fluegge, Daniela ;
Moeller, Lisa M. ;
Cichy, Annika ;
Gorin, Monika ;
Weth, Agnes ;
Veitinger, Sophie ;
Cainarca, Silvia ;
Lohmer, Stefan ;
Corazza, Sabrina ;
Neuhaus, Eva M. ;
Baumgartner, Werner ;
Spehr, Jennifer ;
Spehr, Marc .
NATURE NEUROSCIENCE, 2012, 15 (05) :754-762
[10]   Elucidating the Neuronal Architecture of Olfactory Glomeruli in the Drosophila Antennal Lobe [J].
Grabe, Veit ;
Baschwitz, Amelie ;
Dweck, Hany K. M. ;
Lavista-Llanos, Sofia ;
Hansson, Bill S. ;
Sachse, Silke .
CELL REPORTS, 2016, 16 (12) :3401-3413