Radiation-induced formation of polyacrylic acid-protein covalent conjugates

被引：6
|
作者：
Mustafaev, M [1 ]
Bayülken, S
Ergen, E
Erkol, AY
Ardagil, N
机构：
[1] TUBITAK Marmara Res Ctr, Res Inst Genet Engn & Biotechnol, TR-41470 Gebze Kocaeli, Turkey
[2] Yyldyz Tech Univ, Dept Biochem, Istanbul, Turkey
[3] Cekmece Res & Training Ctr, Dept Chem, TR-34831 Istanbul, Turkey
来源：
RADIATION PHYSICS AND CHEMISTRY | 2001年 / 60卷 / 06期
关键词：
D O I：
10.1016/S0969-806X(00)00423-0
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Radiation-induced formation of water-soluble covalent conjugates of polyacrylic acid (PAA) with negatively charged proteins - bovine serum albumin (BSA) and superoxide dismutase (SOD)- was investigated at neutral pH. Aqueous solutions of PAA, BSA, SOD and their mixtures (PAA-BSA, PAA-Cu2+-BSA, PAA-SOD) have been irradiated with Co-60 gamma -rays and the resulting changes have been measured by high-performance liquid chromatography and UV-Visible spectrophotometry. Crosslinking and formation of water-soluble conjugates of PAA with BSA molecules upon irradiation were observed in the PAA-BSA and PAA-Cu2+ -BSA mixtures. In contrast to these systems, Cu(II), Zn(II) ions in the structure of SOD molecules protect the free protein, and the protein component of PAA-SOD mixtures against radiation damage, and prevent the PAA-SOD covalent conjugate formation. The mechanism of observed protection effect is discussed. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：567 / 575
页数：9
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