Noncovalent and Site-Directed Spin Labeling of Nucleic Acids

被引：40
|
作者：
Shelke, Sandip A. [1 ]
Sigurdsson, Snorri Th. [1 ]
机构：
[1] Univ Iceland, Inst Sci, IS-107 Reykjavik, Iceland
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 43期
关键词：
DNA; EPR spectroscopy; nucleic acids; receptor-ligand interactions; spin labels; SINGLE-NUCLEOTIDE POLYMORPHISMS; ELECTRON DOUBLE-RESONANCE; EPR SPECTROSCOPY; PARAMAGNETIC-RESONANCE; ABASIC SITES; METAL-ION; DNA; RNA; OLIGONUCLEOTIDES; DYNAMICS;
D O I：
10.1002/anie.201002637
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Spin label parking: The rigid spin label ç (magenta), which is an analogue of cytidine, binds site-specifically to abasic sites in duplex DNAs, through hydrogen bonding to guanine (blue) and π stacking with the flanking base pairs. EPR spectroscopy shows complete binding of ç to the abasic site at low temperatures. This new spin-labeling strategy gives easy access to spin-labeled nucleic acids for structural studies by pulsed EPR techniques. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：7984 / 7986
页数：3
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