A light-initiated chemiluminescent assay for the detection of children's milk protein-specific IgE with excellent ability to avoid interference of specific IgG

被引：8

作者：

Zhang, Bei ^{[1
]}

Liu, Dandan ^{[1
]}

Zheng, Lisheng ^{[2
]}

Tan, Xin ^{[1
]}

Yu, Yang ^{[1
]}

Zhang, Jiayi ^{[2
]}

Li, Xue ^{[1
]}

Li, Huiqiang ^{[1
]}

机构：

[1] Tianjin Med Univ, Sch Med Lab, Tianjin, Peoples R China

[2] Tianjin Childrens Hosp, Dept Lab Med, Tianjin, Peoples R China

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2021年 / 497卷

基金：

中国国家自然科学基金;

关键词：

Specific IgE; Specific IgG; Interference; LICA; Cow's milk allergy; COWS MILK; ORAL DESENSITIZATION; ALLERGY; IMMUNOCAP; DIAGNOSIS; IMMUNOASSAYS; ANTIBODY; ANTIGEN; ARRAY;

D O I：

10.1016/j.jim.2021.113110

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The detection of allergen-specific IgE is of value for the diagnosis of children''s milk allergy. However, its accuracy will interfere with the presence of high levels of specific IgG in the serum of children with milk allergy. To solve this problem, we established a light-initiated chemiluminescent assay (LICA) based on nanomicrospheres, which neutralized the interference of specific IgG by increasing the amount of antigen coated on the microspheres. The ability of this method to resist IgG interference was confirmed by adding extra specific IgG to the serum of allergic patients. Finally, the positive rate of allergen-specific IgE was increased to 85%, which was better than the indirect ELISA (70%), indicating that this method has certain advantages for the detection of specific IgE in children with milk allergy.

引用

页数：6

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