Random amplified polymorphic DNA PCR in the teaching of molecular epidemiology

被引：3
|
作者：
Reinoso, Elina B. [1 ]
Bettera, Susana G. [1 ]
机构：
[1] Univ Nacl Rio Cuarto, Fac Ciencias Exactas Fis Quim & Nat, Dept Microbiol & Inmunol, Ruta 36 Km 601,X5804ZAB, Cordoba, Argentina
来源：
BIOCHEMISTRY AND MOLECULAR BIOLOGY EDUCATION | 2016年 / 44卷 / 04期
关键词：
RAPD-PCR; molecular epidemiology; laboratory exercises; STAPHYLOCOCCUS-AUREUS; ARBITRARY PRIMERS; IDENTIFICATION; MILK;
D O I：
10.1002/bmb.20957
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this article, we describe a basic practical laboratory designed for fifth-year undergraduate students of Microbiology as part of the Epidemiology course. This practice provides the students with the tools for molecular epidemiological analysis of pathogenic microorganisms using a rapid and simple PCR technique. The aim of this work was to assay RAPD-PCR technique in order to infer possible epidemiological relationships. The activity gives students an appreciation of the value of applying a simple molecular biological method as RAPD-PCR to a discipline-specific question. It comprises a three-session laboratory module to genetically assay DNAs from strains isolated from a food outbreak. (c) 2016 by The International Union of Biochemistry and Molecular Biology, 44(4):391-396, 2016.
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页码：391 / 396
页数：6
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