Transcriptome Profile Analysis from Different Sex Types of Ginkgo biloba L.

被引：29

作者：

Du, Shuhui ^{[1
]}

Sang, Yalin ^{[1
]}

Liu, Xiaojing ^{[1
]}

Xing, Shiyan ^{[1
]}

Li, Jihong ^{[1
]}

Tang, Haixia ^{[1
]}

Sun, Limin ^{[1
]}

机构：

[1] Shandong Agr Univ, Coll Forestry, Key Lab Sivilculture Shandong Prov, Tai An, Shandong, Peoples R China

来源：

FRONTIERS IN PLANT SCIENCE | 2016年 / 7卷

基金：

中国博士后科学基金;

关键词：

Ginkgo biloba; sex determination; RNA-sequencing; plant hormone signal and transduction; DNA methyltransferase; hydroxysteroid dehydrogenase; DIOECIOUS PLANT; GENE-EXPRESSION; RNA-SEQ; ASPERGILLUS-ORYZAE; GENOME ANNOTATION; DNA METHYLATION; FEMALE FLOWERS; LINKED GENE; SEQUENCE; MELANDRIUM;

D O I：

10.3389/fpls.2016.00871

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In plants, sex determination is a comprehensive process of correlated events, which involves genes that are differentially and/or specifically expressed in distinct developmental phases. Exploring gene expression profiles from different sex types will contribute to fully understanding sex determination in plants. In this study, we conducted RNA-sequencing of female and male buds (FB and MB) as well as ovulate strobilus and staminate strobilus (OS and SS) of Ginkgo biloba to gain insights into the genes potentially related to sex determination in this species. Approximately 60 Gb of clean reads were obtained from eight cDNA libraries. De novo assembly of the clean reads generated 108,307 unigenes with an average length of 796 bp. Among these unigenes, 51,953 (47.97%) had at least one significant match with a gene sequence in the public databases searched. A total of 4709 and 9802 differentially expressed genes (DEGs) were identified in MB vs. FB and SS vs. OS, respectively. Genes involved in plant hormone signal and transduction as well as those encoding DNA methyltransferase were found to be differentially expressed between different sex types. Their potential roles in sex determination of G. biloba were discussed. Pistil-related genes were expressed in male buds while anther-specific genes were identified in female buds, suggesting that dioecism in G. biloba was resulted from the selective arrest of reproductive primordia. High correlation of expression level was found between the RNA-Seq and quantitative real-time PCR results. The transcriptome resources that we generated allowed us to characterize gene expression profiles and examine differential expression profiles, which provided foundations for identifying functional genes associated with sex determination in G. biloba.

引用

页数：10

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