A Sanger sequencing protocol for SARS-CoV-2 S-gene

被引：16
|
作者：
Daniels, Rodney S. [1 ]
Harvey, Ruth [1 ]
Ermetal, Burcu [1 ]
Xiang, Zheng [1 ]
Galiano, Monica [1 ]
Adams, Lorin [1 ]
McCauley, John W. [1 ]
机构：
[1] Worldwide Influenza Ctr, WHO Collaborating Ctr Reference & Res Influenza, Francis Crick Inst, London, England
来源：
INFLUENZA AND OTHER RESPIRATORY VIRUSES | 2021年 / 15卷 / 06期
基金：
英国惠康基金; 英国医学研究理事会;
关键词：
base-calling accuracy; Sanger sequencing; SARS-CoV-2; S-gene; variant detection; NEUTRALIZING ANTIBODY-ACTIVITY;
D O I：
10.1111/irv.12892
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
We describe a Sanger sequencing protocol for SARS-CoV-2 S-gene the Spike (S)-glycoprotein product of which, composed of receptor-binding (S1) and membrane fusion (S2) segments, is the target of vaccines used to combat COVID-19. The protocol can be used in laboratories with basic Sanger sequencing capabilities and allows rapid "at source" screening for SARS-CoV-2 variants, notably those of concern. The protocol has been applied for surveillance, with clinical specimens collected in either nucleic acid preservation lysis-mix or virus transport medium, and research involving cultured viruses, and can yield data of public health importance in a timely manner.
引用
收藏
页码：707 / 710
页数：4
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