A molecular probe based on pyrimidine imidazole derivatives for stable super-resolution endoplasmic reticulum imaging in living cells

被引：5

作者：

Hu, Ling ^{[1
]}

Hussain, Sajid ^{[1
]}

Liu, Tianyan ^{[1
]}

Yue, Yuanzhen ^{[1
]}

Liu, Jiejie ^{[2
]}

Tian, Yupeng ^{[2
]}

Tian, Xiaohe ^{[1
]}

机构：

[1] Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China

[2] Anhui Univ, Dept Chem, Key Lab Funct Inorgan Mat Chem Anhui Prov, Hefei 230601, Anhui, Peoples R China

来源：

NEW JOURNAL OF CHEMISTRY | 2018年 / 42卷 / 18期

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

2-PHOTON ABSORPTION; PHOTODYNAMIC THERAPY; SINGLET OXYGEN; MITOCHONDRIA; MICROSCOPY; DESIGN; DYES;

D O I：

10.1039/c8nj03986k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Multi-functional florescent dyes capable of acting as molecular probes in living systems under two-photon microscopy, as well as super-resolution nanoscopy, are of great interest. Herein, a novel donor--acceptor (D--A) type pyrimidine imidazole derivative with a flexible alkyl chain molecule (EX-1) has been efficiently synthesized through improved Knoevenagel condensation with high yield and relatively large 2PA cross-sections. Furthermore, a cytotoxicity assay and photo-resistance evaluation indicated that such small organic dyes could not only effectively target the endoplasmic reticulum in living cells, but also reveal its ultrastructure under stimulated emission depletion (STED) nanoscopy.

引用

页码：14725 / 14728

页数：4

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