Toxicogenomic responses of human alveolar epithelial cells to tungsten boride nanoparticles

被引:17
作者
Turkez, Hasan [1 ]
Arslan, Mehmet Enes [1 ]
Sonmez, Erdal [2 ,3 ]
Tatar, Abdulgani [4 ]
Acikyildiz, Metin [5 ]
Geyikoglu, Fatime [6 ]
机构
[1] Erzurum Tech Univ, Dept Mol Biol & Genet, Fac Sci, Erzurum, Turkey
[2] Ataturk Univ, Kazim Karabekir Educ Fac, Dept Phys, Erzurum, Turkey
[3] Ataturk Univ, Grad Sch Nat & Appl Sci, Dept Nanosci & Nanoengn, Adv Mat Res Lab, Erzurum, Turkey
[4] Ataturk Univ, Med Fac, Dept Med Genet, Erzurum, Turkey
[5] Kilis 7 Aralik Univ, Fac Sci & Art, Dept Chem, Kilis, Turkey
[6] Ataturk Univ, Fac Arts & Sci, Dept Biol, Erzurum, Turkey
关键词
Tungsten boride nanoparticles; Microarray analysis; Toxicogenomics; Human lung alveolar epithelial cells; IN-VITRO; MECHANISMS; REGULATORS; TOXICITY; REVEALS; GROWTH;
D O I
10.1016/j.cbi.2017.06.027
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
During the recent years, microarray analysis of gene expression has become an inevitable tool for exploring toxicity of drugs and other chemicals on biological systems. Therefore, toxicogenomics is considered as a fruitful area for searching cellular pathways and mechanisms including cancer, immunological diseases, environmental responses, gene-gene interactions and chemical toxicity. In this work, we examined toxic effects of Tungsten Borides NPs on gene expression profiling of the human lung alveolar epithelial cells (HPAEpiC). In line with this purpose, a single crystal of tungsten boride (mixture of WB and W2B) nanoparticles was synthesized by means of zone melting method, and characterized via using X-ray crystallography (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDX) techniques. Cell viability and cytotoxicity were determined by 3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT), neutral red (NR) and lactate dehydrogenase (LDH) release tests. The whole genome microarray expression analysis was performed to find out the effects of WB and W2B NPs mixture on gene expression of the HPAEpiC cell culture. 123 of 40,000 gene probes were assigned to characterize expression profile for WB/W2B NPs exposure. According to results; 70 genes were up-regulated and 53 genes were down-regulated (>= 2 fold change). For further investigations, these genes were functionally classified by using DAVID (The Database for Annotation, Visualization and Integrated Discovery) with gene ontology (GO) analysis. In the light of the data gained from this study, it could be concluded that the mixture of WB/W2B NPs can affect cytokine/chemokine metabolism, angiogenesis and prevent migration/invasion by activating various genes. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:257 / 265
页数:9
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