Effect of storage time on the urine protein: creatinine ratio in alkaline ovine urine

被引:2
|
作者
Athanasiou, Labrini V. [1 ]
Katsoulos, Panagiotis D. [2 ]
Spanou, Victoria M. [1 ]
Pazarakioti, Aikaterini T. [1 ]
Katsogiannou, Eleni G. [1 ]
Iliadi, Ioanna [1 ]
Baka, Rania [3 ]
Polizopoulou, Zoe S. [3 ]
机构
[1] Univ Thessaly, Dept Med, Fac Vet Med, Thessaly, Greece
[2] Aristotle Univ Thessaloniki, Sch Vet Med, Clin Farm Anim, Thessaloniki, Greece
[3] Aristotle Univ Thessaloniki, Sch Vet Med, Diagnost Lab, Thessaloniki, Greece
关键词
alkaline urine; sheep; stability; urine protein; creatinine ratio; VARIABILITY; TEMPERATURE; EXCRETION; SHEEP; PH;
D O I
10.1177/10406387211031517
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The urine protein:creatinine (UPC) ratio is considered the reference method to assess proteinuria. Its diagnostic value in ovine medicine needs further elucidation. In population monitoring and/or for research purposes, it is convenient to collect many samples simultaneously and store them for later analysis. However, analyte stability data are required to ensure reliable results. We used 15 of 90 urine samples collected from sheep to assess the effect of storage time on the UPC ratio. After centrifugation, the supernatant of each sample was divided into 6 aliquots. Urine protein and creatinine concentrations were determined immediately in one aliquot using the pyrogallol red and a modified Jaffe method, respectively. The other aliquots were stored at -18 degrees C. Based on the absence of active sediment, alkaline urine pH, and UPC ratio >= 0.2, we included 15 samples in our study. The UPC ratio was determined in the stored aliquots 2, 7, 14, 21, and 60 d after collection. The data were analyzed with univariate ANOVA. No significant difference was observed in the urinary concentrations of protein, creatinine, and the UPC ratio (0.8 +/- 0.84 in conventional units and 0.09 +/- 0.095 in SI units) among different times (p > 0.05). The UPC ratio remained stable for 2 mo in ovine urine samples stored at -18 degrees C.
引用
收藏
页码:1176 / 1179
页数:4
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