Serially coupled capillary columns supercritical fluid chromatography with midpoint pressure control

被引：2

作者：

Hirata, Y ^{[1
]}

Tsuda, K ^{[1
]}

Imamura, E ^{[1
]}

机构：

[1] Toyohashi Univ Technol, Sch Mat Sci, Toyohashi, Aichi 4418580, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1062卷 / 02期

关键词：

supercritical fluid chromatography; capillary column; serial coupling; selectivity tuning;

D O I：

10.1016/j.chroma.2004.11.051

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two capillary columns of different polarities were coupled in series by means of a coupling restrictor. The pressure of the first column and the midpoint pressure (between the coupling restrictor and the second column) were controlled independently of each other using two pumps. The selectivity of this separation system was highly dependent on the pressure difference and could be continuously changed between those of two columns. The pressure difference could be changed even in course of separation for fine tuning of the selectivity. Several examples were shown to demonstrate the utility of this method. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：269 / 273

页数：5

共 11 条

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