In vivo multiphoton imaging of bile duct ligation

被引：2

作者：

Liu, Yuan ^{[1
]}

Li, Feng-Chieh ^{[1
]}

Chen, Hsiao-Chin

Chang, Po-shou ^{[1
]}

Yang, Shu-Mei ^{[2
]}

Lee, Hsuan-Shu ^{[2
]}

Dong, Chen-Yuan ^{[1
]}

机构：

[1] Natl Taiwan Univ, Dept Phys, Taipei 106, Taiwan

[2] Natl Taiwan Univ, Dept Internal Med, Taipei 100, Taiwan

来源：

MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES VIII | 2008年 / 6860卷

关键词：

multiphoton; bile duct ligation; in vivo; liver;

D O I：

10.1117/12.764264

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Bile is the exocrine secretion of liver and synthesized by hepatocytes. It is drained into duodenum for the function of digestion or drained into gallbladder for of storage. Bile duct obstruction is a blockage in the tubes that carry bile to the gallbladder and small intestine. However, Bile duct ligation results in the changes of bile acids in serum, liver, urine, and feces1, 2. In this work, we demonstrate a novel technique to image this pathological condition by using a newly developed in vivo imaging system, which includes multiphoton microscopy and intravital hepatic imaging chamber. The images we acquired demonstrate the uptake, processing of 6-CFDA in hepatocytes and excretion of CF in the bile canaliculi. In addition to imaging, we can also measure kinetics of the green fluorescence intensity.

引用

页数：7

共 4 条

[1] CHARACTERIZATION OF UPTAKE AND HYDROLYSIS OF FLUORESCEIN DIACETATE AND CARBOXYFLUORESCEIN DIACETATE BY INTRACELLULAR ESTERASES IN SACCHAROMYCES-CEREVISIAE, WHICH RESULT IN ACCUMULATION OF FLUORESCENT PRODUCT [J].