Carboxylic acid reductases enable intramolecular lactamization reactions

被引：14

作者：

Qin, Zongmin ^{[1
,2
]}

Zhang, Xiaohui ^{[2
]}

Sang, Xianke ^{[2
]}

Zhang, Wuyuan ^{[1
,2
,3
]}

Qu, Ge ^{[1
,2
,3
]}

Sun, Zhoutong ^{[1
,2
,3
]}

机构：

[1] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[2] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China

[3] Natl Technol Innovat Ctr Synthet Biol, Tianjin 300308, Peoples R China

来源：

GREEN SYNTHESIS AND CATALYSIS | 2022年 / 3卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Biocatalysis; Lactams; Carboxylic acid reductase; Intramolecular cyclization; Lactamization; ALDEHYDE OXIDOREDUCTASE; GAMMA-LACTAMS; STRAIN; ENZYME;

D O I：

10.1016/j.gresc.2022.05.009

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

As a versatile type of enzyme, carboxylic acid reductases (CAR) can not only reduce various carboxylic acids to aldehydes in cooperation with cofactors ATP and NADPH but also catalyze the synthesis of amides and esters in the absence of NADPH. Here, we report an intramolecular cyclization catalyzed by CAR only with the use of ATP to transform amino acids into diverse lactams, including & gamma;-/& delta;-/& epsilon;-lactams and chiral derivatives thereof. The observed wide substrate scope and selectivity enable potential applications to be implemented. Our results demonstrate that CAR-catalyzed lactamization is a promising approach for the synthesis of chiral lactam compounds under mild conditions, thereby enriching the toolbox for the biosynthesis of lactams as a viable alternative to purely chemical procedures.

引用

页码：294 / 297

页数：4

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