Molecular characterization of Mycoplasma gallisepticum isolates from Turkeys

被引:15
作者
Kleven, SH [1 ]
Fulton, RM
García, M
Ikuta, VN
Leiting, VA
Liu, T
Ley, DH
Opengart, KN
Rowland, GN
Wallner-Pendleton, E
机构
[1] Univ Georgia, Dept Avian Med, Athens, GA 30602 USA
[2] Michigan State Univ, Anim Hlth Diagnost Lab, E Lansing, MI 48824 USA
[3] Simbios Biotecnol, Porto Alegre, RS, Brazil
[4] N Carolina State Univ, Coll Vet Med, Raleigh, NC 27606 USA
[5] Wampler Foods Inc, Harrisonburg, VA 22802 USA
[6] Univ Nebraska, Vet Diagnost Ctr, Lincoln, NE 68583 USA
关键词
Mycoplasma gallisepticum; vaccine; turkey; serology; pathogenesis;
D O I
10.1637/7148
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Mycoplasma gallisepticum was isolated from several turkey flocks at different locations in the United States that were clinically affected with respiratory disease. Five of these isolates from four series of outbreaks had patterns similar to the 6/85 vaccine strain of M gallisepticum by random amplified polymorphic DNA (RAPD) analysis using three different primer sets, whereas with a fourth primer set (OPA13 and OPA14), only two of the isolates were similar to 6/85. Results obtained by sequencing portions of the pvpA, gapA, and mgc2 genes and an uncharacterized surface lipoprotein gene indicated that the field isolates had DNA sequences that ranged from 97.6% to 100%, similar to the 6/85 results. In some of the outbreaks there was an indirect association with the presence of commercial layers in the area that had been vaccinated with this vaccine strain, but there was no known close association with vaccinated birds in any of the outbreaks. Turkeys were challenged with two of the field isolates and with 6/85 vaccine strain. Turkeys challenged with the field isolates developed respiratory disease with airsacculitis and a typical M gallisepticum antibody response, whereas birds challenged with 6/85 developed no respiratory signs or lesions and developed only a weak antibody response. Although these isolates were very similar to the 6/85 vaccine strain, it was not possible to prove that they originated from the vaccine strain-it is possible that they could be naturally occurring field isolates.
引用
收藏
页码:562 / 569
页数:8
相关论文
共 27 条
[1]   Molecular characterization of the Mycoplasma gallisepticum pvpA gene which encodes a putative variable cytadhesin protein [J].
Boguslavsky, S ;
Menaker, D ;
Lysnyansky, I ;
Liu, T ;
Levisohn, S ;
Rosengarten, R ;
García, M ;
Yogev, D .
INFECTION AND IMMUNITY, 2000, 68 (07) :3956-3964
[2]  
Branton SL, 2002, AVIAN DIS, V46, P423, DOI 10.1637/0005-2086(2002)046[0423:TEOLMG]2.0.CO
[3]  
2
[4]   Complementary randomly amplified polymorphic DNA (RAPD) analysis patterns and primer sets to differentiate Mycoplasma gallisepticum strains [J].
Charlton, BR ;
Bickford, AA ;
Walker, RL ;
Yamamoto, R .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1999, 11 (02) :158-161
[5]   EVALUATION OF A MYCOPLASMA GALLISEPTICUM STRAIN EXHIBITING REDUCED VIRULENCE FOR PREVENTION AND CONTROL OF POULTRY MYCOPLASMOSIS [J].
EVANS, RD ;
HAFEZ, YS .
AVIAN DISEASES, 1992, 36 (02) :197-201
[6]   DEMONSTRATION OF THE GENETIC STABILITY OF A MYCOPLASMA-GALLISEPTICUM STRAIN FOLLOWING INVIVO PASSAGE [J].
EVANS, RD ;
HAFEZ, YS ;
SCHREURS, CS .
AVIAN DISEASES, 1992, 36 (03) :554-560
[7]   Application of polymerase chain reaction with arbitrary primers to strain identification of Mycoplasma gallisepticum [J].
Fan, HH ;
Kleven, SH ;
Jackwood, MW .
AVIAN DISEASES, 1995, 39 (04) :729-735
[8]   MYCOPLASMA-GALLISEPTICUM STRAIN DIFFERENTIATION BY ARBITRARY PRIMER PCR (RAPD) FINGERPRINTING [J].
GEARY, SJ ;
FORSYTH, MH ;
SAOUD, SA ;
WANG, G ;
BERG, DE ;
BERG, CM .
MOLECULAR AND CELLULAR PROBES, 1994, 8 (04) :311-316
[9]   Molecular and biochemical analysis of a 105 kDa Mycoplasma gallisepticum cytadhesin (GapA) [J].
Goh, MS ;
Gorton, TS ;
Forsyth, MH ;
Troy, KE ;
Geary, SJ .
MICROBIOLOGY-SGM, 1998, 144 :2971-2978
[10]   Characterization of MGC2, a Mycoplasma gallisepticum cytadhesin with homology to the Mycoplasma pneumoniae 30-kilodalton protein P30 and Mycoplasma genitalium P32 [J].
Hnatow, LL ;
Keeler, CL ;
Tessmer, LL ;
Czymmek, K ;
Dohms, JE .
INFECTION AND IMMUNITY, 1998, 66 (07) :3436-3442