Development and validation of an analytical method for detection of estrogens in water

被引：52
作者：
Noppe, H
De Wasch, K
Poelmans, S
Van Hoof, N
Verslycke, T
Janssen, CR
De Brabander, H
机构：
[1] Univ Ghent, Fac Med Vet, Dept Vet Publ Hlth & Food Safety, Lab Chem Anal, B-9820 Merelbeke, Belgium
[2] Woods Hole Oceanog Inst, Dept Biol, Woods Hole, MA 02543 USA
[3] Univ Ghent, Fac Biosci Engn, Lab Environm Toxicol & Aquat Ecol, B-9000 Ghent, Belgium
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2005年 / 382卷 / 01期
关键词：
endocrine disruption; environmental estrogens; Scheldt estuary; validation; water analysis;
D O I：
10.1007/s00216-005-3174-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An analytical procedure enabling routine analysis of four environmental estrogens at concentrations below 1 ng L-1 in estuarine water samples has been developed and validated. The method includes extraction of water samples using solid-phase extraction discs and detection by gas chromatography (GC) with tandem mass spectrometry (MS-MS) in electron-impact (EI) mode. The targeted estrogens included 17 alpha- and 17 beta-estradiol (aE2, bE2), estrone (E1), and 17 alpha-ethinylestradiol (EE2), all known environmental endocrine disruptors. Method performance characteristics, for example trueness, recovery, calibration, precision, accuracy, limit of quanti. cation (LOQ), and the stability of the compounds are presented for each of the selected estrogens. Application of the procedure to water samples from the Scheldt estuary (Belgium - The Netherlands), a polluted estuary with reported incidences of environmental endocrine disruption, revealed that E1 was detected most frequently at concentrations up to 7 ng L-1. aE2 was detected once only and concentrations of bE2 and EE2 were below the LOQ.
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页码：91 / 98
页数：8
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