Effect of Hydrogen-Rich Water on Radiation-Induced Cognitive Dysfunction in Rats

被引：14
作者：
Liu, Mengya [1 ]
Yuan, Hui [1 ]
Yin, Jingjing [1 ]
Wang, Ruoqi [1 ]
Song, Jianbo [2 ]
Hu, Bo [1 ]
Li, Jianguo [1 ]
Qin, Xiujun [1 ]
机构：
[1] China Inst Radiat Protect, Dept Radiol & Environm Med, 102 Xuefu Rd, Taiyuan 030006, Peoples R China
[2] Shanxi Dayi Hosp, Dept Radiotherapy, Taiyuan 030032, Peoples R China
来源：
RADIATION RESEARCH | 2020年 / 193卷 / 01期
关键词：
HIPPOCAMPAL NEUROGENESIS IMPAIRMENT; OXIDATIVE STRESS; IRRADIATION; EXERCISE; DEFICITS; SALINE;
D O I：
10.1667/RR15464.1
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The goal of this work was to determine whether hydrogen-rich water (HRW) could attenuate radiation-induced cognitive dysfunction in rats and to explore the underlying mechanisms. Rats received 30 Gy whole-brain irradiation using a 6-MeV electron beam. Either purified water or HRW (0.8-0.9 ppm) was administrated at 10 min prior to irradiation, as well as a daily HRW treatment after irradiation for 30 consecutive days. The Morris water maze was used to test spatial memory in the rats. The concentration of glutathione (GSH), malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OHdG) and the super-oxidedismutase (SOD) activity in cerebral cortex, as well as brain-derived neurotrophic factor (BDNF) level in serum, were measured. Immunofluorescence staining was adopted to detect proliferating cells. The expression of BDNF-TrkB pathway-related genes and proteins were detected using qRT-PCR and Western blot. Models of cognitive dysfunction were successfully established using a 30 Gy dose of ionizing radiation. Compared to the radiation treated group, the radiation-HRW treated group showed significantly decreased escape latency (P < 0.05), but increased retention time, swimming distance of original platform quadrant (P < 0.05) and number of platform crossings (P < 0.05). Furthermore, the SOD, GSH (P < 0.05) and BDNF (P < 0.05) levels in the radiation-HRW treated group were higher compared to the radiation treated group. The MDA and 8OHdG levels (P < 0.05) were decreased in the radiationHRW treated group when compared to the radiation treated group. Additionally, treatment with HRW increased the number of BrdU(+)NeuN(+) cells in the radiation treated group. The mRNA and protein levels of BDNF and TrkB (P < 0.05) in radiation-HRW treated group was higher than that in the radiation treated group. Collectively, our study indicates that HRW has a protective effect on radiation-induced cognitive dysfunction, and that the possible mechanisms mainly involve anti-oxidative and anti-inflammatory reactions, and its protection of newborn neurons by regulating the BDNF-TrkB signaling pathway. (C) 2020 by Radiation Research Society
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页码：16 / 23
页数：8
相关论文
共 29 条
[1]   PURIFICATION OF A NEW NEUROTROPHIC FACTOR FROM MAMMALIAN BRAIN [J].
BARDE, YA ;
EDGAR, D ;
THOENEN, H .
EMBO JOURNAL, 1982, 1 (05) :549-553
[2]   Hydrogen therapy reduces apoptosis in neonatal hypoxia-ischemia rat model [J].
Cai, Jianmei ;
Kang, Zhimin ;
Liu, Wen Wu ;
Luo, Xu ;
Qiang, Sun ;
Zhang, John H. ;
Ohta, Shigeo ;
Sun, Xuejun ;
Xu, Weigang ;
Tao, Hengyi ;
Li, Runping .
NEUROSCIENCE LETTERS, 2008, 441 (02) :167-172
[3]   Fe-56 particle exposure results in a long-lasting increase in a cellular index of genomic instability and transiently suppresses adult hippocampal neurogenesis in vivo [J].
DeCarolis, Nathan A. ;
Rivera, Phillip D. ;
Ahn, Francisca ;
Amaral, Wellington Z. ;
LeBlanc, Junie A. ;
Malhotra, Shveta ;
Shih, Hung-Ying ;
Petrik, David ;
Melvin, Neal R. ;
Chen, Benjamin P. C. ;
Eisch, Amelia J. .
LIFE SCIENCES IN SPACE RESEARCH, 2014, 2 :70-79
[4]  
Gosselin Tracy K, 2002, Clin J Oncol Nurs, V6, P175, DOI 10.1188/02.CJON.175-176
[5]   Inhalation of hydrogen gas elevates urinary 8-hydroxy-2 '-deoxyguanine in Parkinson's disease [J].
Hirayama, Masaaki ;
Ito, Mikako ;
Minato, Tomomi ;
Yoritaka, Asako ;
LeBaron, Tyler W. ;
Ohno, Kinji .
MEDICAL GAS RESEARCH, 2018, 8 (04) :144-+
[6]   Beneficial biological effects and the underlying mechanisms of molecular hydrogen - comprehensive review of 321 original articles [J].
Ichihara, Masatoshi ;
Sobue, Sayaka ;
Ito, Mikako ;
Ito, Masafumi ;
Hirayama, Masaaki ;
Ohno, Kinji .
MEDICAL GAS RESEARCH, 2015, 5
[7]   Effects of paraoxon on serum biochemical parameters and oxidative stress induction in various tissues of Wistar and Norway rats [J].
Jafari, Mahvash ;
Salehi, Maryam ;
Asgari, Alireza ;
Ahmadi, Sediq ;
Abbasnezhad, Maryam ;
Hajihoosani, Reza ;
Hajigholamali, Mansoure .
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2012, 34 (03) :876-887
[8]   Forced running exercise attenuates hippocampal neurogenesis impairment and the neurocognitive deficits induced by whole-brain irradiation via the BDNF-mediated pathway [J].
Ji, Jian-feng ;
Ji, Sheng-jun ;
Sun, Rui ;
Li, Kun ;
Zhang, Yuan ;
Zhang, Li-yuan ;
Tian, Ye .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2014, 443 (02) :646-651
[9]   Irradiation-induced hippocampal neurogenesis impairment is associated with epigenetic regulation of bdnf gene transcription [J].
Ji, Shengjun ;
Tian, Ye ;
Lu, Yi ;
Sun, Rui ;
Ji, Jianfeng ;
Zhang, Liyuan ;
Duan, Shumin .
BRAIN RESEARCH, 2014, 1577 :77-88
[10]   Loss of hippocampal neurogenesis, increased novelty-induced activity, decreased home cage activity, and impaired reversal learning one year after irradiation of the young mouse brain [J].
Kalm, Marie ;
Karlsson, Niklas ;
Nilsson, Marie K. L. ;
Blomgren, Klas .
EXPERIMENTAL NEUROLOGY, 2013, 247 :402-409
123