Transmembrane adaptor protein WBP1L regulates CXCR4 signalling and murine haematopoiesis

被引：8

作者：

Borna, Simon ^{[1
,2
]}

Drobek, Ales ^{[1
]}

Kralova, Jarmila ^{[1
]}

Glatzova, Daniela ^{[1
,2
,3
]}

Splichalova, Iva ^{[4
]}

Fabisik, Matej ^{[1
,2
]}

Pokorna, Jana ^{[1
]}

Skopcova, Tereza ^{[1
]}

Angelisova, Pavla ^{[1
]}

Kanderova, Veronika ^{[5
,6
]}

Starkova, Julia ^{[5
,6
]}

Stanek, Petr ^{[7
]}

Matveichuk, Orest, V ^{[8
]}

Pavliuchenko, Nataliia ^{[1
,2
]}

Kwiatkowska, Katarzyna ^{[8
]}

Protty, Majd B. ^{[9
]}

Tomlinson, Michael G. ^{[10
]}

Alberich-Jorda, Meritxell ^{[11
]}

Korinek, Vladimir ^{[12
]}

Brdicka, Tomas ^{[1
]}

机构：

[1] Czech Acad Sci, Inst Mol Genet, Lab Leukocyte Signaling, Prague, Czech Republic

[2] Charles Univ Prague, Fac Sci, Prague, Czech Republic

[3] Czech Acad Sci, J Heyrovsky Inst Phys Chem, Dept Biophys Chem, Prague, Czech Republic

[4] Czech Acad Sci, Inst Mol Genet, Lab Immunobiol, Prague, Czech Republic

[5] Charles Univ Prague, Fac Med 2, Childhood Leukaemia Invest Prague, Prague, Czech Republic

[6] Charles Univ Prague, Fac Med 2, Dept Pediat Hematol & Oncol, Prague, Czech Republic

[7] Charles Univ Prague, Fac Med 2, Prague, Czech Republic

[8] Nencki Inst Expt Biol, Lab Mol Membrane Biol, Warsaw, Poland

[9] Univ Birmingham, Inst Biomed Res, Birmingham, W Midlands, England

[10] Univ Birmingham, Sch Biosci, Birmingham, W Midlands, England

[11] Czech Acad Sci, Inst Mol Genet, Lab Hematooncol, Prague, Czech Republic

[12] Czech Acad Sci, Inst Mol Genet, Lab Cell & Dev Biol, Prague, Czech Republic

来源：

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | 2020年 / 24卷 / 02期

基金：

欧盟地平线“2020”; 英国医学研究理事会;

关键词：

bone marrow homing; bone marrow transplantation; CXCR4; ETV6; haematopoiesis; haematopoietic stem cell; NEDD4; family; OPAL1; RUNX1; WBP1L; WHIM-SYNDROME; GENE; EXPRESSION; LEUKEMIA; UBIQUITIN; ETV6; TEL/ETV6; TEL;

D O I：

10.1111/jcmm.14895

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

WW domain binding protein 1-like (WBP1L), also known as outcome predictor of acute leukaemia 1 (OPAL1), is a transmembrane adaptor protein, expression of which correlates with ETV6-RUNX1 (t(12;21)(p13;q22)) translocation and favourable prognosis in childhood leukaemia. It has a broad expression pattern in haematopoietic and in non-haematopoietic cells. However, its physiological function has been unknown. Here, we show that WBP1L negatively regulates signalling through a critical chemokine receptor CXCR4 in multiple leucocyte subsets and cell lines. We also show that WBP1L interacts with NEDD4-family ubiquitin ligases and regulates CXCR4 ubiquitination and expression. Moreover, analysis of Wbp1l-deficient mice revealed alterations in B cell development and enhanced efficiency of bone marrow cell transplantation. Collectively, our data show that WBP1L is a novel regulator of CXCR4 signalling and haematopoiesis.

引用

页码：1980 / 1992

页数：13

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